ArticleScientific reports2021
High engraftment capacity of frozen ready-to-use human fecal microbiota transplants assessed in germ-free mice.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Transplant of cryopreserved mosquito gut microbiota reveals strain-specific patterns of microbial acquisition and assembly.ISME host microbe · 2026Article
- Anorexia nervosa symptoms are induced after specific gut microbiota dysbiosis transfer in germ-free mice.Gut microbes · 2025Article
- Mouse strain-specific responses along the gut-brain axis upon fecal microbiota transplantation from children with autism.Gut microbes · 2025Article
- Article
- Trehalose and maltodextrin preserve microbial community structure in freeze-dried fecal samples for fecal microbiota transplantation.ISME communications · 2025Article
- Freeze-dried fecal microorganisms as an effective biomaterial for the treatment of calves suffering from diarrhea.Scientific reports · 2024Article
- Investigation of Freezing and Freeze-Drying for Preserving and Re-Using a Whole Microbial Cheese Community.Foods (Basel, Switzerland) · 2024Article
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Authors and funding
15 authors.
Funding
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Abstract
The number of indications for fecal microbiota transplantation is expected to rise, thus increasing the needs for production of readily available frozen or freeze-dried transplants. Using shotgun metagenomics, we investigated the capacity of two novel human fecal microbiota transplants prepared in maltodextrin-trehalose solutions (abbreviated MD and TR for maltodextrin:trehalose, 3:1, w/w, and trehalose:maltodextrin 3:1, w/w, respectively), to colonize a germ-free born mouse model. Gavage with frozen-thawed MD or TR suspensions gave the taxonomic profiles of mouse feces that best resembled those obtained with the fresh inoculum (Spearman correlations based on relative abundances of metagenomic species around 0.80 and 0.75 for MD and TR respectively), while engraftment capacity of defrosted NaCl transplants most diverged (Spearman correlations around 0.63). Engraftment of members of the family Lachnospiraceae and Ruminoccocaceae was the most challenging in all groups of mice, being improved with MD and TR transplants compared to NaCl, but still lower than with the fresh preparation. Improvement of engraftment of this important group in maintaining health represents a challenge that could benefit from further research on fecal microbiota transplant manufacturing.
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