ArticleBiotechnology for biofuels2021
Acetate as substrate for L-malic acid production with Aspergillus oryzae DSM 1863.
Article in Biotechnology for biofuels, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- In Situ Imaging for Monitoring Biomass Content and Pellet Size in Submerged Filamentous Stirred-Tank Cultivation.Biotechnology journal · 2026Article
- Understanding the Role of pH Regulation and Neutralizing Agents in Organic Acid Production and Growth of Aspergillus oryzae.Biotechnology and bioengineering · 2026Article
- Transcriptome analysis of Aspergillus oryzae RIB40 under chemical stress reveals mechanisms of adaptation to fungistatic compounds of lignocellulosic side streams.Biotechnology for biofuels and bioproducts · 2025Article
- Two-stage conversion of syngas and pyrolysis aqueous condensate into L-malate.Biotechnology for biofuels and bioproducts · 2024Article
- The postbiotic potential ofFrontiers in microbiology · 2024Review
- Perspectives for Using COBioengineering (Basel, Switzerland) · 2023Review
- Recent advances in producing food additive L-malate: Chassis, substrate, pathway, fermentation regulation and application.Microbial biotechnology · 2023Review
- Microbial cell factories based on filamentous bacteria, yeasts, and fungi.Microbial cell factories · 2023Review
- Metabolic engineering using acetate as a promising building block for the production of bio-based chemicals.Engineering microbiology · 2022Review
- Optimization of L-malic acid production from acetate with Aspergillus oryzae DSM 1863 using a pH-coupled feeding strategy.Microbial cell factories · 2022Article
- RNA-Seq Based Transcriptome Analysis ofJournal of fungi (Basel, Switzerland) · 2022Article
- Detoxification of a pyrolytic aqueous condensate from wheat straw for utilization as substrate in Aspergillus oryzae DSM 1863 cultivations.Biotechnology for biofuels and bioproducts · 2022Article
- Effect of process mode, nitrogen source and temperature on L-malic acid production withFrontiers in bioengineering and biotechnology · 2022Article
- Enhanced l-Malic Acid Production byFrontiers in bioengineering and biotechnology · 2021Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundMicrobial malic acid production is currently not able to compete economically with well-established chemical processes using fossil resources. The utilization of inexpensive biomass-based substrates containing acetate could decrease production costs and promote the development of microbial processes. Acetate is a by-product in lignocellulosic hydrolysates and fast pyrolysis products or can be synthesized by acetogens during syngas fermentation. For the fermentation of these substrates, a robust microorganism with a high tolerance for biomass-derived inhibitors is required. Aspergillus oryzae is a suitable candidate due to its high tolerance and broad substrate spectrum. To pave the path towards microbial malic acid production, the potential of acetate as a carbon source for A. oryzae is evaluated in this study.
resultsA broad acetate concentration range was tested both for growth and malic acid production with A. oryzae. Dry biomass concentration was highest for acetic acid concentrations of 40-55 g/L reaching values of about 1.1 g/L within 48 h. Morphological changes were observed depending on the acetate concentration, yielding a pellet-like morphology with low and a filamentous structure with high substrate concentrations. For malic acid production, 45 g/L acetic acid was ideal, resulting in a product concentration of 8.44 ± 0.42 g/L after 192 h. The addition of 5-15 g/L glucose to acetate medium proved beneficial by lowering the time point of maximum productivity and increasing malic acid yield. The side product spectrum of cultures with acetate, glucose, and cultures containing both substrates was compared, showing differences especially in the amount of oxalic, succinic, and citric acid produced. Furthermore, the presence of CaCO
conclusionsThis study evaluates relevant aspects of malic acid production with A. oryzae using acetate as carbon source and demonstrates that it is a suitable substrate for biomass formation and acid synthesis. The insights provided here will be useful to further microbial malic acid production using renewable substrates.
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