ArticlePlant methods2021
Optimization of protoplast regeneration in the model plant Arabidopsis thaliana.
Article in Plant methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- A positive feedback loop between NPR1 and SOG1 reinforces genotoxic tolerance in Arabidopsis.Plant communications · 2026Article
- An effective method for isolation and regeneration of Solanum tuberosum mesophyll protoplasts for transgene-free genome editing.Journal, genetic engineering & biotechnology · 2026Article
- Genome-wide identification of the MYB gene family and identification of candidate genes that involved in anthocyanin synthesis in cowpea (Vigna unguiculata [L.] Walp).BMC plant biology · 2026Article
- Stem cell activity is linked to a transient acquisition of pluripotency during callus proliferation.Nature communications · 2026Article
- Protoplasts Isolation and Transient Transformation System Optimization for Poplar 84K (Biology · 2026Article
- Cell-type-specific gating of gene regulatory modules as a hallmark of early immune responses in Arabidopsis leaves.The New phytologist · 2026Article
- TOP1α-mediated stabilization of CYP82C4 G-quadruplex DNA represses lateral root primordium initiation in Arabidopsis.Nature plants · 2026Article
- Crop biofortification for global food security: advances in genetic engineering and biotechnological approaches.Plant molecular biology · 2026Review
- Protoplast-Based Functional Genomics and Genome Editing: Progress, Challenges and Applications.Plant, cell & environment · 2026Review
- Red and Far-Red LED Lighting Enhances Protoplast-to-Plant Regeneration in Broccoli (Plants (Basel, Switzerland) · 2026Article
- Molecular Regulators of In Vitro Regeneration in Wheat: Roles of Morphogenic Factors in Transformation, Genome Editing, and Breeding.International journal of molecular sciences · 2026Review
- Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering.Plant biotechnology journal · 2026Article
- Aggregates-on-chip: a microfluidic device for crowding plant cells in culture.Frontiers in plant science · 2026Article
- CRISPR/Cas9-Targeted Gene Editing of Allergenic Profilin-EncodingPlants (Basel, Switzerland) · 2025Article
- Impedance flow cytometry for rapid quality assessment of protoplast cultures.Plant methods · 2025Article
- PVP-40 mediated enhancement of mesophyll protoplast yield and viability for transient gene expression in black huckleberry.Plant methods · 2025Article
- Using a Supramolecular Approach to Engineer Modular Hydrogel Platforms for Culturing Protoplasts - from General Tissue Engineering to Cellular Agriculture.Advanced biology · 2025Article
- Establishing a reliable protoplast system for grapevine: isolation, transformation, and callus induction.Protoplasma · 2025Article
- Stable transgene expression and CRISPR-mediated knock-in system of a bacteria-derived antibiotic selection gene in the green alga Ulva prolifera.BMC plant biology · 2025Article
- Identification of optimal adenine and cytosine base editors for genome editing in Arabidopsis and soybean.BMB reports · 2025Article
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Authors and funding
5 authors.
Funding
Abstract
backgroundPlants have a remarkable reprogramming potential, which facilitates plant regeneration, especially from a single cell. Protoplasts have the ability to form a cell wall and undergo cell division, allowing whole plant regeneration. With the growing need for protoplast regeneration in genetic engineering and genome editing, fundamental studies that enhance our understanding of cell cycle re-entry, pluripotency acquisition, and de novo tissue regeneration are essential. To conduct these studies, a reproducible and efficient protoplast regeneration method using model plants is necessary.
resultsHere, we optimized cell and tissue culture methods for improving protoplast regeneration efficiency in Arabidopsis thaliana. Protoplasts were isolated from whole seedlings of four different Arabidopsis ecotypes including Columbia (Col-0), Wassilewskija (Ws-2), Nossen (No-0), and HR (HR-10). Among these ecotypes, Ws-2 showed the highest potential for protoplast regeneration. A modified thin alginate layer was applied to the protoplast culture at an optimal density of 1 × 10
conclusionThe cell and tissue culture system optimized in this study for protoplast regeneration is efficient and reproducible. This method of Arabidopsis protoplast regeneration can be used for fundamental studies on pluripotency establishment and de novo tissue regeneration.
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