Evidence map›Paper›PMID 33620593›Full record

ArticleJournal, genetic engineering & biotechnology2021

Gnotobiotic evaluation of Dalbergia sissoo genotypes for resistance against Fusarium solani via dual culture set up.

Monika Chauhan, Ajay Thakur, Yashaswi Singh, M Maqbool Rather, Nirmal S K Harsh

Open access · diamondAbstract read
In one paragraph

Article in Journal, genetic engineering & biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Monika ChauhanTissue Culture Laboratory, Genetics and Tree Improvement Division, Forest Research Institute, Dehra Dun, Uttarakhand, 248006, India.
Ajay ThakurTissue Culture Laboratory, Genetics and Tree Improvement Division, Forest Research Institute, Dehra Dun, Uttarakhand, 248006, India. mithoojorhat@yahoo.co.in.ORCID http://orcid.org/0000-0002-2309-0905
Yashaswi SinghTissue Culture Laboratory, Genetics and Tree Improvement Division, Forest Research Institute, Dehra Dun, Uttarakhand, 248006, India.ORCID https://orcid.org/0000-0002-7390-2561
M Maqbool RatherFaculty of Forestry, SKUAST-Kashmir, Benehama, Ganderbal, Jammu and Kashmir, India.
Nirmal S K HarshForest Pathology Division, Forest Research Institute, Dehra Dun, Uttarakhand, 248006, India.
Forest Research Institute · INSher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDalbergia sissoo (shisham), an important multipurpose tree native to the Indian subcontinent and also planted in other countries, has been afflicted with large scale mortality in all age groups due to wilt disease, causing huge economic losses. Fusarium solani f. sp. dalbergiae (Fsd) has been identified as one of the causal organisms for wilt disease in D. sissoo. One of the approaches of disease resistance studies involves co-cultivation of trees and pathogens under controlled conditions to screen resistant tree genotypes. A gnotobiotic condition, where the pathogen is known, enables accurate screening of disease-resistant genotypes. In the present study, ten genotypes of D. sissoo were cloned in vitro and evaluated against two strains of Fsd in a dual culture setup under gnotobiotic conditions with an objective to identify resistant genotypes of D. sissoo against Fsd.

resultsCallus and plantlets of ten genotypes of host plant multiplied in vitro were inoculated with conidial suspension of two strains of Fsd at three concentrations; 1 × 10

conclusionGnotobiotic evaluation of callus and plantlets of ten genotypes of D. sissoo against Fsd strains has reduced time and space otherwise required for field trials. Genetic variations amongst the genotypes resulted in varying responses towards virulent Fsd strains and only two out of ten genotypes showed promising resistant characteristics. In dual culture setup, both callus and plantlets of the same genotypes responded similarly against Fsd strains, which signify that in vitro screening can be used as an indirect selection method for disease resistance.

Indexed as

Fusarium solani f. sp. dalbergiaeFusarium wiltGenetic variationGnotobiotaIn vitro screeningPlant-microbe interactionShisham mortality

Identifiers

PMID33620593
PMCPMC7902750
OpenAlexW3132124148

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.