ArticlePlant physiology2021
OsmiR396/growth regulating factor modulate rice grain size through direct regulation of embryo-specific miR408.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 86 citations in OpenAlex.
- The ZmmiR169o/ZmNF-YA13 module orchestrates maize kernel texture by fine-tuning starch biosynthesis and endosperm architecture.BMC plant biology · 2026Article
- The multifaceted regulatory network of microRNA396: Orchestrating plant plasticity through coordinating development and various stresses.Journal of integrative plant biology · 2026Review
- miR408-5p and miR408-3p cooperatively reduce cadmium uptake and accumulation in rice.PLoS biology · 2026Article
- QTL analysis of sorghum grain traits based on high-density genetic map.Journal of applied genetics · 2025Article
- The genome of giant waterlily provides insights into the origin of angiosperms, leaf gigantism, and stamen function innovation.Plant communications · 2025Article
- Non-coding RNAs and their role in plants: prospective omics-tools for improving growth, development and stress tolerance in field crops.Molecular biology reports · 2025Review
- The TCP transcription factor TAC8 positively regulates the tiller angle in rice (Oryza sativa L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- The Integrated Analysis of miRNome and Degradome Sequencing Reveals the Regulatory Mechanisms of Seed Development and Oil Biosynthesis in Pecan (Foods (Basel, Switzerland) · 2024Article
- Gγ-protein GS3 Function in Tight Genetic Relation with OsmiR396/GS2 to Regulate Grain Size in Rice.Rice (New York, N.Y.) · 2024Article
- Article
- Article
- Modulation of histone acetylation enables fully mechanized hybrid rice breeding.Nature plants · 2024Article
- OsmiR5519 regulates grain size and weight and down-regulates sucrose synthase gene RSUS2 in rice (Oryza sativa L.).Planta · 2024Article
- Genome-wide identification of the GRF family in sweet orange (Citrus sinensis) and functional analysis of the CsGRF04 in response to multiple abiotic stresses.BMC genomics · 2024Article
- Article
- Genetic Basis of Grain Size and Weight in Rice, Wheat, and Barley.International journal of molecular sciences · 2023Review
- The miR167-OsARF12 module regulates rice grain filling and grain size downstream of miR159.Plant communications · 2023Article
- Joint analysis of phenotype-effect-generation identifies loci associated with grain quality traits in rice hybrids.Nature communications · 2023Article
- OsmiR159 Modulate BPH Resistance Through Regulating G-Protein γ Subunit GS3 Gene in Rice.Rice (New York, N.Y.) · 2023Article
- Riboflavin mediates m6A modification targeted by miR408, promoting early somatic embryogenesis in longan.Plant physiology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
microRNAs (miRNAs) are promising targets for crop improvement of complex agricultural traits. Coordinated activity between/among different miRNAs may fine-tune specific developmental processes in diverse organisms. Grain size is a main factor determining rice (Oryza sativa L.) crop yield, but the network of miRNAs influencing this trait remains uncharacterized. Here we show that sequestering OsmiR396 through target mimicry (MIM396) can substantially increase grain size in several japonica and indica rice subspecies and in plants with excessive tillers and a high panicle density. Thus, OsmiR396 has a major role related to the regulation of rice grain size. The grain shape of Growth Regulating Factor8 (OsGRF8)-overexpressing transgenic plants was most similar to that of MIM396 plants, suggesting OsGRF8 is a major mediator of OsmiR396 in grain size regulation. A miRNA microarray analysis revealed changes to the expression of many miRNAs, including OsmiR408, in the MIM396 plants. Analyses of gene expression patterns and functions indicated OsmiR408 is an embryo-specific miRNA that positively regulates grain size. Silencing OsmiR408 expression (miR408KO) using CRISPR technology resulted in small grains. Moreover, we revealed the direct regulatory effects of OsGRF8 on OsMIR408 expression. A genetic analysis further showed that the large-grain phenotype of MIM396 plants could be complemented by miR408KO. Also, several hormone signaling pathways might be involved in the OsmiR396/GRF-meditated grain size regulation. Our findings suggest that genetic regulatory networks comprising various miRNAs, such as OsmiR396 and OsmiR408, may be crucial for controlling rice grain size. Furthermore, the OsmiR396/GRF module may be important for breeding new high-yielding rice varieties.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.