ArticleOrthopaedic surgery2021
CircCDR1as Suppresses Bone Microvascular Endothelial Cell Activity and Angiogenesis Through Targeting miR-135b/ FIH-1 Axis.
Article in Orthopaedic surgery, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 14 citations in OpenAlex.
- Diagnostic biomarkers for non-traumatic osteonecrosis of the femoral head : a systematic review and pathophysiological cascade network.Bone & joint research · 2026Pooled it
- Epigenetic dysregulation in osteonecrosis of the femoral head: a critical review of DNA methylation, histone modifications, and clinical translation.Journal of orthopaedic surgery and research · 2026Review
- Advances in the mechanism for steroid-induced osteonecrosis of the femoral head.Bone research · 2026Review
- Functional nucleic acid Hydrogels: Paving the way for Next-generation bone and cartilage regeneration.Materials today. Bio · 2026Review
- Cardiac Circular RNAs CDR1as, Circ-RCAN2, Circ-C12orf29 Show Cell-Specific Hypoxia-Induced Dysregulation and Distinct In Vitro Effects.International journal of molecular sciences · 2025Article
- Emerging perspectives on osteonecrosis of the femoral head: the role of circular RNAs and long non-coding RNAs - a systematic review.Frontiers in genetics · 2025Review
- Identification of circRNA CDR1as/miR-214-3p regulatory axis in Legg-Calvé-Perthes disease.Orphanet journal of rare diseases · 2024Article
- miR-135b: An emerging player in cardio-cerebrovascular diseases.Journal of pharmaceutical analysis · 2024Review
- Article
- Unique miRomics Expression Profiles inInternational journal of molecular sciences · 2023Article
- Article
- Steroid-Induced Osteonecrosis of the Femoral Head: Novel Insight Into the Roles of Bone Endothelial Cells in Pathogenesis and Treatment.Frontiers in cell and developmental biology · 2021Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe current study investigated the role of CircCDR1as on angiogenesis of bone microvascular endothelial cells (BMECs) isolated from non-traumatic ONFH.
methodsForty corticosteroid-induced ONFH patients received THA were enrolled in our study. Expressions of CircCDR1as, miR-135b, and FIH-1 were detected by qRT-PCR in affected necrosis tissue and non-affected normal tissue. Bone microvascular endothelial cells (BMEC) were isolated from six patients and treated with 0.1 mg/mL hydrocortisone to establish a GC-damaged model of BMECs. Circ CDR1as plasmid and miR-135b mimic were transfected into BMECs. BMEC proliferation was assessed using MTT assays. The migration ability of cells was detected by scratch-wound assays. Matrigel assay was performed to detect angiogenesis in vitro. Western blot assay was used to detect HIF-1α, VEGF, and FIH-1 expressions. FISH, RNA pull down, RIP, and luciferase assay were carried out to determine the interaction of CircCDR1as, miR-135b, and FIH-1.
resultsCircCDR1as was upregulated(2.02 ± 0.30 vs. 1.00 ± 0.10,P < 0.001) whereas miR-135b was downregulated (0.55 ± 0.12 vs. 1.00 ± 0.10,P < 0.001) in affected tissues than in non-affected tissues. Expression of CircCDR1as and FIH-1 were negatively associated with miR-135b in affected tissues (CircCDR1as with miR-135b: r = -0.506, P < 0.001; FIH-1 with miR-135b r = -0.510, P < 0.001). Total blood tubule density was increased when CircCDR1as was silenced compared with NC (P < 0.01 vs. NC). The number of migrated BMECs were significantly increased in CircCDR1as silencing group compared with NC group (P < 0.05 vs. NC). In addition, CircCDR1as plasmids transfection increased the protein expressions of FIH-1 (P < 0.05 vs. NC) and reduced the HIF-1α as well as VEGF expression compared with NC group (P < 0.05 vs. NC). FISH, RNA pull down, RIP, and luciferase assay identified that FIH-1 was a target of miR-135b and could be modulated by CircCDR1as.
conclusionCircCDR1as decreases angiogenesis and proliferation of BMECs by sponging miR-135b and upregulate FIH-1.
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