ArticleJournal of assisted reproduction and genetics2021
Centriolar defects, centrin 1 alterations, and FISH studies in human spermatozoa of a male partner of a couple that produces aneuploid embryos in natural and artificial fertilization.
Article in Journal of assisted reproduction and genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 9 citations in OpenAlex.
- Human Sperm Centrosome: From Current Evidence to Future Perspectives-A Systematic Review.Life (Basel, Switzerland) · 2026Review
- Molecular models of the sperm head-tail coupling apparatus.Journal of cell science · 2025Review
- The relevance of sperm morphology in male infertility.Frontiers in reproductive health · 2022Article
- Single cell analysis of DNA in more than 10,000 individual sperm from men with abnormal reproductive outcomes.Journal of assisted reproduction and genetics · 2021Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo study the potential paternal contribution to aneuploidies in the man of a couple who obtained trisomic embryos with natural and assisted fertilization.
methodsSemen analysis, immunofluorescence for localization of tubulin and centrin 1, transmission electron microscopy (TEM), and fluorescence in situ hybridization (FISH) analysis for chromosomes 18 and 9 were performed. Sperm of fertile men were used as controls.
resultsThe percentages of sperm motility and normal forms were decreased. The percentages of sperm with tail reduced in dimension, headless tails, coiled tails, and altered head-tail junction were significantly higher (P < 0.01) in the patient than in controls, whereas the percentage of sperm with a normal centrin 1 localization (two spots in the centriolar area) was significantly reduced (P < 0.01) in the patient. Immunofluorescence with anti-tubulin antibody showed that in most of the patient's sperm connecting pieces (83.00 ± 1.78%), two spots were present, indicating prominent proximal centriole/centriolar adjunct and evident distal centriole, whereas controls' sperm displayed a single spot, indicating the proximal centriole. The percentage of sperm with two spots was significantly higher (P < 0.01) in the patient than in controls. TEM analysis showed that centriolar adjuncts of the patient's sperm were significantly longer (721.80 ± 122.26 nm) than in controls' sperm (310.00 ± 64.11 nm; P < 0.001). The aneuploidy frequencies of the patient's sperm, detected by FISH analysis, were increased with respect to controls.
conclusionA paternal contribution to sperm aneuploidies cannot be excluded since the patient's sperm showed altered morphology, immature centriolar adjunct, presence of evident distal centriole, scarce presence of centrin 1, and high aneuploidy frequency.
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