ReviewProtoplasma2021
Three-dimensional quantitative imaging of Tobacco mosaic virus and Zucchini yellow mosaic virus induced ultrastructural changes.
Review in Protoplasma, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- Integration of whey and mycorrhizal symbiosis: a sustainable biocontrol strategy against Zucchini yellow mosaic virus in squash.Mycorrhiza · 2026Article
- Plantorganelle Hunter is an effective deep-learning-based method for plant organelle phenotyping in electron microscopy.Nature plants · 2023Article
- Organelle-specific localization of glutathione in plants grown under different light intensities and spectra.Histochemistry and cell biology · 2022Review
- Volumetric 3D reconstruction of plant leaf cells using SEM, ion milling, TEM, and serial sectioning.Planta · 2022Article
- Image-Based Analysis Revealing the Molecular Mechanism of Peroxisome Dynamics in Plants.Frontiers in cell and developmental biology · 2022Review
- Ultrastructure of plant cells.Protoplasma · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two-dimensional ultrastructural changes of Tobacco mosaic virus (TMV) and Zucchini yellow mosaic virus (ZYMV) in tobacco and pumpkin plants, respectively, are well studied. To provide 3D data, representative control and infected cells were reconstructed using serial sectioning and transmission electron microscopy. Quantitative data of 3D ultrastructural changes were then extracted from the cytosol and organelles by image analysis. While TMV induced the accumulation of an average of 40 virus inclusion bodies in the cytosol, which covered about 13% of the cell volume, ZYMV caused the accumulation of an average of 1752 cylindrical inclusions in the cytosol, which covered about 2.7% of the total volume of the cell. TMV infection significantly decreased the number and size of mitochondria (- 49 and - 20%) and peroxisomes (- 62 and - 28%) of the reconstructed cell. The reconstructed ZYMV-infected cell contained more (105%) and larger (109%) mitochondria when compared to the control cell. While the reconstructed TMV-infected cell contained larger (20%) and the ZYMV-infected smaller (19%) chloroplasts, both contained less chloroplasts (- 40% for TMV and - 23% for ZYMV). In chloroplasts, the volume of starch and plastoglobules increased (664% and 150% for TMV and 1324% and 1300% for ZYMV) when compared to the control. The latter was correlated with a decrease in the volume of thylakoids in the reconstructed ZYMV-infected cell (- 31%) indicating that degradation products from thylakoids are transported and stored in plastoglobules. Summing up, the data collected in this study give a comprehensive overview of 3D changes induced by TMV and ZYMV in plants.
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Registered trials
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