Evidence map›Paper›PMID 33619503›Full record

ArticlemedRxiv : the preprint server for health sciences2021

Saliva TwoStep for rapid detection of asymptomatic SARS-CoV-2 carriers.

Qing Yang, Nicholas R Meyerson, Stephen K Clark, Camille L Paige, Will T Fattor, Alison R Gilchrist, Arturo Barbachano-Guerrero, Benjamin G Healy, Emma R Worden-Sapper, Sharon S Wu and 15 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in medRxiv : the preprint server for health sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 31 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

25 authors at 2 institutions in 1 country.

Qing YangBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
Nicholas R MeyersonBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
Stephen K ClarkBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
Camille L PaigeBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
Will T FattorBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
Alison R GilchristBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
Arturo Barbachano-GuerreroBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
Benjamin G HealyDepartment of Mechanical Engineering, University of Colorado Boulder, Boulder, Colorado, 80303.
Emma R Worden-SapperBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
Sharon S WuBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
Denise MuhlradDepartment of Biochemistry, University of Colorado Boulder, Boulder, Colorado, 80303.
Carolyn J DeckerDepartment of Biochemistry, University of Colorado Boulder, Boulder, Colorado, 80303.
Tassa K SaldiBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
Erika LasdaBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
Patrick K GonzalesBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
Morgan R FinkBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
Kimngan L TatBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
Cole R HagerBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
Jack C DavisDepartment of Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Boulder, Colorado, 80303.
Christopher D OzeroffBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
Gloria R BrissonWardenburg Health Center, University of Colorado Boulder, Boulder, Colorado, 80303.
Matthew B McQueenDepartment of Integrative Physiology, University of Colorado Boulder, Boulder, Colorado, 80303.
Leslie LeinwandBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
Roy ParkerBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
Sara L SawyerBioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, 80303.
University of Colorado Boulder · USHoward Hughes Medical Institute · US

Funding

Hunting the HIV-1 UnicornDP1DA046108 · NIDA · UNIVERSITY OF COLORADO · PI SAWYER, SARA · 2018 to 2022
$4.5M
NIDA NIH HHS DP1 DA046108
6 · The paper itself

Abstract

Here, we develop a simple molecular test for SARS-CoV-2 in saliva based on reverse transcription loop-mediated isothermal amplification (RT-LAMP). The test has two steps: 1) heat saliva with a stabilization solution, and 2) detect virus by incubating with a primer/enzyme mix. After incubation, saliva samples containing the SARS-CoV-2 genome turn bright yellow. Because this test is pH dependent, it can react falsely to some naturally acidic saliva samples. We report unique saliva stabilization protocols that rendered 295 healthy saliva samples compatible with the test, producing zero false positives. We also evaluated the test on 278 saliva samples from individuals who were infected with SARS-CoV-2 but had no symptoms at the time of saliva collection, and from 54 matched pairs of saliva and anterior nasal samples from infected individuals. The Saliva TwoStep test described herein identified infections with 94% sensitivity and >99% specificity in individuals with sub-clinical (asymptomatic or pre-symptomatic) infections.

Identifiers

PMID33619503
PMCPMC7899473
OpenAlexW3129847635

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.