Evidence map›Paper›PMID 33619372›Full record

ArticleScientific reports2021

Graphene oxide assisted light-up aptamer selection against Thioflavin T for label-free detection of microRNA.

Md Mamunul Islam, Victoria Michele Ghielmetti, Peter B Allen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Md Mamunul IslamDepartment of Chemistry, 001 Renfrew Hall, University of Idaho, 875 Perimeter Dr, Moscow, ID, 83844-2343, USA.
Victoria Michele GhielmettiDepartment of Chemistry, 001 Renfrew Hall, University of Idaho, 875 Perimeter Dr, Moscow, ID, 83844-2343, USA.
Peter B AllenDepartment of Chemistry, 001 Renfrew Hall, University of Idaho, 875 Perimeter Dr, Moscow, ID, 83844-2343, USA. pballen@uidaho.edu.
University of Idaho · US

Funding

Genomics Resources Core (GRC)P30GM103324 · NIGMS · UNIVERSITY OF IDAHO · PI SETTLES, MATTHEW · 2013 to 2017
$5.0M
NIGMS NIH HHS P30 GM103324
6 · The paper itself

Abstract

We selected an aptamer against a fluorogenic dye called Thioflavin T (ThT). Aptamers are single-stranded DNA that can bind a specific target. We selected the ThT aptamer using graphene oxide assisted SELEX and a low-cost Open qPCR instrument. We optimized, minimized, and characterized the best aptamer candidate against ThT. The aptamer, ThT dye, and the enzymatic strand displacement amplification (SDA) were used in a label-free approach to detect the micro RNA miR-215 in saliva and serum. The aptamer confers higher specificity than intercalating dyes but without expensive covalently modified DNA probes. This isothermal, low-cost, simple method can detect both DNA and RNA. The target, miR-215, was detected with a limit of detection of 2.6 nM.

Indexed as

Aptamers, NucleotideBenzothiazolesBiosensing TechniquesBase SequenceDNADNA ProbesFluorescent DyesGraphiteMicroRNAsNanotechnologySensitivity and SpecificitySpectrum AnalysisAptamers, NucleotideBenzothiazolesDNADNA ProbesFluorescent Dyesgraphene oxideGraphiteMicroRNAsthioflavin T

Identifiers

PMID33619372
PMCPMC7900183
OpenAlexW3129876376

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.