ArticleScientific reports2021
Single-cell RNA sequencing reveals developmental heterogeneity among Plasmodium berghei sporozoites.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Demystifying Plasmodium development within the Anopheles mosquito.Current opinion in microbiology · 2026Review
- Single-cell profiling reveals immunoregulation of artemisinin on CD8Innovation (Cambridge (Mass.)) · 2025Article
- Novel single-cell preservation and RNA sequencing technology unlocks field studies for Plasmodium natural infections.Scientific reports · 2025Article
- Parasite and vector circadian clocks mediate efficient malaria transmission.Nature microbiology · 2025Article
- The spatiotemporal transcriptional profiling of murine brain during cerebral malaria progression and after artemisinin treatment.Nature communications · 2025Article
- Genomic advances in the study of the mosquito vector during avian malaria infection.Parasitology · 2023Review
- AnPathogens and global health · 2023Article
- Long read single cell RNA sequencing reveals the isoform diversity of Plasmodium vivax transcripts.PLoS neglected tropical diseases · 2022Article
- Comparative single-cell transcriptional atlases of Babesia species reveal conserved and species-specific expression profiles.PLoS biology · 2022Article
- Single-cell RNA sequencing of Plasmodium vivax sporozoites reveals stage- and species-specific transcriptomic signatures.PLoS neglected tropical diseases · 2022Article
- MAEBL Contributes toInternational journal of molecular sciences · 2022Article
- Plasmodium development in Anopheles: a tale of shared resources.Trends in parasitology · 2022Review
- Single-cell RNA profiling ofFrontiers in cellular and infection microbiology · 2022Article
- Liver-stage fate determination inFrontiers in microbiology · 2022Article
- Unraveling the intricacies of host-pathogen interaction through single-cell genomics.Microbes and infectionReview
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the malaria-causing parasite's life cycle, Plasmodium sporozoites must travel from the midgut of a mosquito to the salivary glands before they can infect a mammalian host. However, only a fraction of sporozoites complete the journey. Since salivary gland invasion is required for transmission of sporozoites, insights at the molecular level can contribute to strategies for malaria prevention. Recent advances in single-cell RNA sequencing provide an opportunity to assess sporozoite heterogeneity at a resolution unattainable by bulk RNA sequencing methods. In this study, we use a droplet-based single-cell RNA sequencing workflow to analyze the transcriptomes of over 8000 Plasmodium berghei sporozoites derived from the midguts and salivary glands of Anopheles stephensi mosquitoes. The detection of known marker genes confirms the successful capture and sequencing of samples composed of a mixed population of sporozoites. Using data integration, clustering, and trajectory analyses, we reveal differences in gene expression profiles of individual sporozoites, and identify both annotated and unannotated markers associated with sporozoite development. Our work highlights the utility of a high-throughput workflow for the transcriptomic profiling of Plasmodium sporozoites, and provides new insights into gene usage during the parasite's development in the mosquito.
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