Evidence map›Paper›PMID 33615605›Full record

ArticleCell proliferation2021

PAK4 suppresses motor neuron degeneration in hSOD1

Chaohua Cong, Weiwei Liang, Chunting Zhang, Ying Wang, Yueqing Yang, Xudong Wang, Shuyu Wang, Di Huo, Hongyong Wang, Di Wang and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell proliferation, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.1field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 13 citations in OpenAlex.

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  4. Rho family GTPase signaling through type II p21-activated kinases.Cellular and molecular life sciences : CMLS · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Chaohua CongDepartment of Neurology, The First Clinical College of Harbin Medical University, Harbin, China.
Weiwei LiangDepartment of Neurology, The First Clinical College of Harbin Medical University, Harbin, China.
Chunting ZhangDepartment of Neurology, The First Clinical College of Harbin Medical University, Harbin, China.
Ying WangDepartment of Neurology, The First Clinical College of Harbin Medical University, Harbin, China.
Yueqing YangDepartment of Neurology, The Second Clinical College of Harbin Medical University, Harbin, China.
Xudong WangDepartment of Neurology, The First Clinical College of Harbin Medical University, Harbin, China.
Shuyu WangDepartment of Neurology, The First Clinical College of Harbin Medical University, Harbin, China.
Di HuoDepartment of Neurology, The First Clinical College of Harbin Medical University, Harbin, China.
Hongyong WangDepartment of Neurology, The First Clinical College of Harbin Medical University, Harbin, China.
Di WangDepartment of Neurology, The First Clinical College of Harbin Medical University, Harbin, China.
Honglin FengDepartment of Neurology, The First Clinical College of Harbin Medical University, Harbin, China.ORCID https://orcid.org/0000-0002-5062-8846
Harbin Medical University · CN

Funding

Innovative Research Program for Graduates of Harbin Medical University YJSKYCX2018-32HYDNational Natural Science Foundation of China 81571227National Natural Science Foundation of China 91849133
6 · The paper itself

Abstract

objectivesAmyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the progressive loss of motor neurons (MN). CREB pathway-mediated inhibition of apoptosis contributes to neuron protection, and PAK4 activates CREB signalling in diverse cell types. This study aimed to investigate PAK4's effect and mechanism of action in ALS.

methodsWe analysed RNA levels by qRT-PCR, protein levels by immunofluorescence and Western blotting, and apoptosis by flow cytometry and TUNEL staining. Cell transfection was performed for in vitro experiment. Mice were injected intraspinally to evaluate PAK4 function in vivo experiment. Rotarod test was performed to measure motor function.

resultsThe expression and activation of PAK4 significantly decreased in the cell and mouse models of ALS as the disease progressed, which was caused by the negative regulation of miR-9-5p. Silencing of PAK4 increased the apoptosis of MN by inhibiting CREB-mediated neuroprotection, whereas overexpression of PAK4 protected MN from hSOD1

conclusionsPAK4 protects MN from degeneration by activating the anti-apoptotic effects of CREB signalling, suggesting it may be a therapeutic target in ALS.

Indexed as

Amyotrophic Lateral SclerosisAnimalsApoptosisCell Line, TumorCREB-Binding ProteinDisease Models, AnimalFemaleMiceMice, Inbred C57BLMice, TransgenicMicroRNAsMotor NeuronsMutagenesis, Site-DirectedNeuroprotective Agentsp21-Activated KinasesRNA InterferenceCREB-Binding ProteinCrebbp protein, mouseMicroRNAsMIRN9 microRNA, mouseNeuroprotective Agentsp21-Activated KinasesPak4 protein, mouseRNA, Small InterferingSuperoxide Dismutase-1

Identifiers

PMID33615605
PMCPMC8016643
OpenAlexW3133012518

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.