ArticleSmall methods2021
Virus-Free and Live-Cell Visualizing SARS-CoV-2 Cell Entry for Studies of Neutralizing Antibodies and Compound Inhibitors.
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Article in Small methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22citing papers in PubMed
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Who cites it
22 citing papers in PubMed, 30 citations in OpenAlex.
- C-terminus-outward orientation of SARS-CoV-2 envelope proteins on viral capsid enables a novel virus-cell interaction pathway.Frontiers in cellular and infection microbiology · 2026Article
- T cell receptor-like antibody specifically targets and eliminates cells infected with cytomegalovirus.Journal of translational medicine · 2025Article
- A quadri-fluorescence SARS-CoV-2 pseudovirus system for efficient antigenic characterization of multiple circulating variants.Cell reports methods · 2024Article
- Antiviral Nanobiologic Therapy Remodulates Innate Immune Responses to Highly Pathogenic Coronavirus.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- COVID-19 Biogenesis and Intracellular Transport.International journal of molecular sciences · 2023Review
- Lineage-mosaic and mutation-patched spike proteins for broad-spectrum COVID-19 vaccine.Cell host & microbe · 2022Article
- Cell-based reporter assays for measurements of antibody-mediated cellular cytotoxicity and phagocytosis against SARS-CoV-2 spike protein.Journal of virological methods · 2022Article
- PIKfyve inhibitors against SARS-CoV-2 and its variants including Omicron.Signal transduction and targeted therapy · 2022Article
- ACE2-enriched extracellular vesicles enhance infectivity of live SARS-CoV-2 virus.Journal of extracellular vesicles · 2022Article
- Cross-species tropism and antigenic landscapes of circulating SARS-CoV-2 variants.Cell reports · 2022Article
- Diltiazem inhibits SARS-CoV-2 cell attachment and internalization and decreases the viral infection in mouse lung.PLoS pathogens · 2022Article
- Receptor-binding domain-anchored peptides block binding of severe acute respiratory syndrome coronavirus 2 spike proteins with cell surface angiotensin-converting enzyme 2.Frontiers in microbiology · 2022Article
- A Bacterially Expressed SARS-CoV-2 Receptor Binding Domain Fused With Cross-Reacting Material 197 A-Domain Elicits High Level of Neutralizing Antibodies in Mice.Frontiers in microbiology · 2022Article
- Imaging Techniques: Essential Tools for the Study of SARS-CoV-2 Infection.Frontiers in cellular and infection microbiology · 2022Review
- Review
- SARS-CoV-2 infection and disease outcomes in non-human primate models: advances and implications.Emerging microbes & infections · 2021Review
- Cell entry by SARS-CoV-2.Trends in biochemical sciences · 2021Review
- A recombinant spike protein subunit vaccine confers protective immunity against SARS-CoV-2 infection and transmission in hamsters.Science translational medicine · 2021Article
- A new high-content screening assay of the entire hepatitis B virus life cycle identifies novel antivirals.JHEP reports : innovation in hepatology · 2021Article
- Viral infection neutralization tests: A focus on severe acute respiratory syndrome-coronavirus-2 with implications for convalescent plasma therapy.Reviews in medical virology · 2021Review
Corrections and comments
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Authors and funding
32 authors at 3 institutions in 1 country.
Yali ZhangState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Shaojuan WangState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Yangtao WuState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Wangheng HouState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Lunzhi YuanState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Chenguang ShenShenzhen Key Laboratory of Pathogen and Immunity National Clinical Research Center for Infectious Disease Shenzhen Third People's Hospital Second Hospital Affiliated to Southern University of Science and Technology Shenzhen Guangdong 518112 China.
Juan WangState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Jianghui YeState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Qingbing ZhengState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Jian MaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Jingjing XuDepartment of Hematology Fujian Medical University Union Hospital Fujian Provincial Key Laboratory on Hematology Fujian Institute of Hematology Fuzhou Fujian 350001 China.
Min WeiState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Zonglin LiState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Sheng NianState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Hualong XiongState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Liang ZhangState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Yang ShiState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Baorong FuState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Jiali CaoState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Chuanlai YangState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Zhiyong LiThe First Hospital of Xiamen University Xiamen 361003 China.
Ting YangDepartment of Hematology Fujian Medical University Union Hospital Fujian Provincial Key Laboratory on Hematology Fujian Institute of Hematology Fuzhou Fujian 350001 China.
Lei LiuShenzhen Key Laboratory of Pathogen and Immunity National Clinical Research Center for Infectious Disease Shenzhen Third People's Hospital Second Hospital Affiliated to Southern University of Science and Technology Shenzhen Guangdong 518112 China.
Hai YuState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Jianda HuDepartment of Hematology Fujian Medical University Union Hospital Fujian Provincial Key Laboratory on Hematology Fujian Institute of Hematology Fuzhou Fujian 350001 China.
Shengxiang GeState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Yixin ChenState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Tianying ZhangState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Jun ZhangState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Tong ChengState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Quan YuanState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.
Ningshao XiaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health & School of Life Sciences Xiamen University Xiamen Fujian 361102 China.ORCID https://orcid.org/0000-0003-0179-5266
Xiamen University · CNFujian Medical University · CNSouthern University of Science and Technology · CN
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ongoing corona virus disease 2019 (COVID-19) pandemic, caused by SARS-CoV-2 infection, has resulted in hundreds of thousands of deaths. Cellular entry of SARS-CoV-2, which is mediated by the viral spike protein and ACE2 receptor, is an essential target for the development of vaccines, therapeutic antibodies, and drugs. Using a mammalian cell expression system, a genetically engineered sensor of fluorescent protein (Gamillus)-fused SARS-CoV-2 spike trimer (STG) to probe the viral entry process is developed. In ACE2-expressing cells, it is found that the STG probe has excellent performance in the live-cell visualization of receptor binding, cellular uptake, and intracellular trafficking of SARS-CoV-2 under virus-free conditions. The new system allows quantitative analyses of the inhibition potentials and detailed influence of COVID-19-convalescent human plasmas, neutralizing antibodies and compounds, providing a versatile tool for high-throughput screening and phenotypic characterization of SARS-CoV-2 entry inhibitors. This approach may also be adapted to develop a viral entry visualization system for other viruses.
Indexed as
entry inhibitorfluorescent proteinhigh‐content analysislive‐cell imagingSARS‐CoV‐2spike glycoproteinviral entry visualization
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