ArticleFrontiers in veterinary science2020
Novel Application of Nanofluidic Chip Digital PCR for Detection of African Swine Fever Virus.
Article in Frontiers in veterinary science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 18 citations in OpenAlex.
- A Triplex Crystal Digital RT-PCR for the Detection of Avian Leukosis Virus, Chicken Infectious Anemia Virus, and Fowl Adenovirus.Animals : an open access journal from MDPI · 2026Article
- Advancements in Modern Nucleic Acid-Based Multiplex Testing Methodologies for the Diagnosis of Swine Infectious Diseases.Veterinary sciences · 2025Review
- Development and validation of a novel triplex droplet digital PCR assay for simultaneous detection of African swine fever virus, pseudorabies virus, and porcine parvovirus.Frontiers in microbiology · 2025Article
- Comparison of digital PCR and real time PCR methods for quantitative analysis of African Swine Fever Virus.Frontiers in veterinary science · 2025Article
- Establishment and application of a dual chip digital PCR assay for detection of PDCoV and PEDV.Frontiers in veterinary science · 2025Article
- Strategic nucleic acid detection approaches for diagnosing African swine fever (ASF): navigating disease dynamics.Veterinary research · 2024Review
- Development of a triplex crystal digital RT-PCR for the detection of PHEV, PRV, and CSFV.Frontiers in veterinary science · 2024Article
- A triplex crystal digital PCR for the detection of genotypes I and II African swine fever virus.Frontiers in veterinary science · 2024Article
- Triplex Crystal Digital PCR for the Detection and Differentiation of the Wild-Type Strain and the MGF505-2R and I177L Gene-Deleted Strain of African Swine Fever Virus.Pathogens (Basel, Switzerland) · 2023Article
- Improvement of Sensitivity and Speed of Virus Sensing Technologies Using nm- and μm-Scale Components.Sensors (Basel, Switzerland) · 2023Review
- Non-nucleic acid extraction and ultra-sensitive detection of African swine fever virus via CRISPR/Cas12a.Applied microbiology and biotechnology · 2022Article
- One-pot platform for rapid detecting virus utilizing recombinase polymerase amplification and CRISPR/Cas12a.Applied microbiology and biotechnology · 2022Article
- A Multiplex Crystal Digital PCR for Detection of African Swine Fever Virus, Classical Swine Fever Virus, and Porcine Reproductive and Respiratory Syndrome Virus.Frontiers in veterinary science · 2022Article
- Development and Application of a Duplex Droplet Digital Polymerase Chain Reaction Assay for Detection and Differentiation of EP402R-Deleted and Wild-Type African Swine Fever Virus.Frontiers in veterinary science · 2022Article
- Micro/nanotechnology-inspired rapid diagnosis of respiratory infectious diseases.Biomedical engineering letters · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
African swine fever virus (ASFV) gives rise to a grievous transboundary and infectious disease, African swine fever (ASF), which has caused a great economic loss in the swine industry. To prevent and control ASF, once suspicious symptoms have presented, the movement of animal and pork products should be stopped, and then, laboratory testing should be adopted to diagnose ASF. A method for ASFV DNA quantification is presented in this research, which utilizes the next-generation PCR platform, nanofluidic chip digital PCR (cdPCR). The cdPCR detection showed good linearity and repeatability. The limit of detection for cdPCR is 30.1995 copies per reaction, whereas no non-specific amplification curve was found with other swine viruses. In the detection of 69 clinical samples, the cdPCR showed significant consistency [91.30% (63/69)] to the Office International des Epizooties-approved quantitative PCR. Compared with the commercial quantitative PCR kit, the sensitivity of the cdPCR assay was 86.27% (44/50), and the specificity was 94.44% (17/18). The positive coincidence rate of the cdPCR assay was 88% (44/50). The total coincidence rate of the cdPCR and kit was 89.86% (62/69), and the kappa value reached 0.800 (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.