ArticleEcology and evolution2021
Biosurveillance for invasive insect pest species using an environmental DNA metabarcoding approach and a high salt trap collection fluid.
Article in Ecology and evolution, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 41 citations in OpenAlex.
- Non-Destructive Detection of Soybean Pest Based on Hyperspectral Image and Attention-ResNet Meta-Learning Model.Sensors (Basel, Switzerland) · 2023Article
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- Reliability and Utility of Standard Gene Sequence Barcodes for the Identification and Differentiation of Cyst Nematodes of the GenusJournal of nematology · 2022Article
- The evolutionary dynamics of biological invasions: A multi-approach perspective.Evolutionary applications · 2021Review
- Validation of an Effective Protocol forInsects · 2021Article
- Optimization and validation of a cost-effective protocol for biosurveillance of invasive alien species.Ecology and evolution · 2021Article
- Biosurveillance for invasive insect pest species using an environmental DNA metabarcoding approach and a high salt trap collection fluid.Ecology and evolution · 2021Article
- Molecular Acquisition, Cleaning and Evaluation in R (MACER) - A tool to assemble molecular marker datasets from BOLD and GenBank.Biodiversity data journal · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the increase in global trade and warming patterns, the movement, introduction, and establishment of non-native insect species has increased. A rapid and effective early detection biosurveillance program to identify species of concern is needed to reduce future impacts and costs associated with introduced non-native species. One of the challenges facing insect surveillance trapping methods is the sheer volume of individual specimens in the collections. Although molecular identification methods are improving, they currently have limitations (e.g., destructive processing of specimens) and a protocol addressing these limitations can support regulatory applications that need morphological evidence to corroborate molecular data.The novel protocol presented here uses a metabarcoding approach to amplify environmental DNA from a saturated salt solution trap fluid, which retains trap specimens for downstream morphological identifications. The use of a saturated salt solution to preserve specimens in traps addresses issues with the high evaporation rate of ethanol in traps, and public safety concerns with other fluid preservation options with unattended traps in public settings.Using a metabarcoding approach, a 407-nucleotide segment of the cytochrome
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.