ArticleAdvanced functional materials2020
Transient Support from Fibroblasts is Sufficient to Drive Functional Vascularization in Engineered Tissues.
Article in Advanced functional materials, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
51 citing papers in PubMed, 75 citations in OpenAlex.
- Self-Organized Vascularized Human Liver Spheroids: Serum-Free Culture Conditions and Use as Tissue Building Blocks.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- A 3D microfluidic model of exchange between perfused blood and lymphatic microvascular networks.Lab on a chip · 2026Article
- Review
- Electrical Stimulation Directs Formation of Perfused Vasculature in Engineered Tissues.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The Role of Endothelial Cell-Derived Extracellular Vesicles in Modulating Fibroblast Function in Skin Wound Healing.The Journal of investigative dermatology · 2026Article
- Development of a customizable vascularization strategy using a modular microfluidic platform for fibroblast-free vascularized tissue.Materials today. Bio · 2026Article
- Vascularized Cardiac Tissue Engineering: From Advances in Biofabrication to Translational Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Coupling intraoperative bioprinting and surgical micropuncture for synergistic scaffold vascularization.Biomaterials · 2026Article
- Modeling fatty liver disease and progression with stem cell derived hepatocytes.Scientific reports · 2026Article
- Three-dimensional modeling of flow through microvascular beds and surrounding interstitial spaces.Bioengineering & translational medicine · 2026Article
- Using High-Throughput Screening to Identify Crosslinking Peptides That Control Cell-Mediated Matrix Degradation.Advanced healthcare materials · 2025Article
- Endothelial-smooth muscle microgauges for modeling pulmonary arterial vasoregulation.Lab on a chip · 2025Article
- Engineering a heparin-mimetic biomaterial to promote tissue vascularization.Communications biology · 2025Article
- Augmented 3D Printing for Multiscale Microphysiological Systems.Small (Weinheim an der Bergstrasse, Germany) · 2025Review
- Progress in recapitulating morphogenesis of blood microvascular structures for microphysiological systems development.Biochemical Society transactions · 2025Article
- Nanosilicates promote angiogenesis through activation of ROS-mediated WNT/β-catenin pathway.Science advances · 2025Article
- Clickable PEG-norbornene microgels support suspension bioprinting and microvascular assembly.Acta biomaterialia · 2025Article
- Semi-synthetic fibrous fibrin composites promote 3D microvascular assembly, survival, and host integration of endothelial cells without mesenchymal cell support.Bioactive materials · 2025Article
- Advances and challenges in human 3D solid tumor models.Advanced functional materials · 2025Article
- Enhancing Viability in Static and Perfused 3D Tissue Constructs Using Sacrificial Gelatin Microparticles.ACS biomaterials science & engineering · 2025Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Formation of capillary blood vasculature is a critical requirement for native as well as engineered organs and can be induced in vitro by co-culturing endothelial cells with fibroblasts. However, whether these fibroblasts are required only in the initial morphogenesis of endothelial cells or needed throughout is unknown, and the ability to remove these stromal cells after assembly could be useful for clinical translation. In this study, we introduce a technique termed CAMEO (Controlled Apoptosis in Multicellular Tissues for Engineered Organogenesis), whereby fibroblasts are selectively ablated on demand, and utilize it to probe the dispensability of fibroblasts in vascular morphogenesis. The presence of fibroblasts is shown to be necessary only during the first few days of endothelial cell morphogenesis, after which they can be ablated without significantly affecting the structural and functional features of the developed vasculature. Furthermore, we demonstrate the use of CAMEO to vascularize a construct containing primary human hepatocytes that improved tissue function. In conclusion, this study suggests that transient, initial support from fibroblasts is sufficient to drive vascular morphogenesis in engineered tissues, and this strategy of engineering-via-elimination may provide a new general approach for achieving desired functions and cell compositions in engineered organs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.