Evidence map›Paper›PMID 33610603›Full record

ArticleExperimental eye research2021

Super-resolution imaging of flat-mounted whole mouse cornea.

Zhen Cai, Yang Zhang, Zheyuan Zhang, Ki-Hee Song, Lisa Beckmann, Ali Djalilian, Cheng Sun, Hao F Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental eye research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Zhen CaiDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
Yang ZhangDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
Zheyuan ZhangDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
Ki-Hee SongDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
Lisa BeckmannDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
Ali DjalilianDepartment of Ophthalmology, University of Illinois Chicago, Chicago, IL, 60612, USA.
Cheng SunDepartment of Mechanical Engineering, Northwestern University, Evanston, IL, 60208, USA.
Hao F ZhangDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA. Electronic address: hfzhang@northwestern.edu.

Funding

TR&D Project 3: Photoacoustic Detection of Metal Fluxes at the Tissue LevelP41GM135018 · NIGMS · NORTHWESTERN UNIVERSITY · PI JACOBSEN, CHRIS JOHNSON, O'HALLORAN, THOMAS V · 2020 to 2024
$5.8M
ER Stress and Diabetic RetinopathyR01EY019949 · NEI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI ZHANG, SARAH X · 2010 to 2023
$5.5M
Investigating oxygen metabolism in diabetic retinopathyR01EY026078 · NEI · NORTHWESTERN UNIVERSITY · PI SHEIBANI, NADER, ZHANG, HAO F · 2016 to 2020
$2.3M
Investigating nanoscale neuronal damages in early glaucoma towards clinical optical detectionR01EY029121 · NEI · NORTHWESTERN UNIVERSITY · PI LIU, XIAORONG, ZHANG, HAO F · 2018 to 2021
$2.0M
Mechanisms underlying the formation of the cornea and ocular surface epitheliumR01EY028304 · NEI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI KUME, TSUTOMU · 2018 to 2021
$1.6M
Visual Sciences T32T32EY025202 · NEI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI Gregory William Schwartz · 2015 to 2026
$1.4M
Intrinsic-contrast super-resolution imaging of DNA at 2-nm resolutionR01GM139151 · NIGMS · NORTHWESTERN UNIVERSITY · PI ZHANG, HAO F · 2020 to 2023
$1.3M
Development of spectroscopic Single-Molecule Localization MicroscopyR01GM140478 · NIGMS · NORTHWESTERN UNIVERSITY · PI SUN, CHENG, ZHANG, HAO F · 2021 to 2024
$1.2M
NEI NIH HHS R01 EY019949NEI NIH HHS R01 EY026078NEI NIH HHS R01 EY028304NEI NIH HHS R01 EY029121NEI NIH HHS T32 EY025202NIGMS NIH HHS P41 GM135018NIGMS NIH HHS R01 GM139151NIGMS NIH HHS R01 GM140478
6 · The paper itself

Abstract

Super-resolution microscopy revolutionized biomedical research with significantly improved imaging resolution down to the molecular scale. To date, only limited studies reported multi-color super-resolution imaging of thin tissue slices mainly because of unavailable staining protocols and incompatible imaging techniques. Here, we show the first super-resolution imaging of flat-mounted whole mouse cornea using single-molecule localization microscopy (SMLM). We optimized immunofluorescence staining protocols for β-Tubulin, Vimentin, Peroxisome marker (PMP70), and Histone-H4 in whole mouse corneas. Using the optimized staining protocols, we imaged these four intracellular protein structures in the epithelium and endothelium layers of flat-mounted mouse corneas. We also achieved simultaneous two-color spectroscopic SMLM (sSMLM) imaging of β-Tubulin and Histone-H4 in corneal endothelial cells. The spatial localization precision of sSMLM in these studies was around 20-nm. This work sets the stage for investigating multiple intracellular alterations in corneal diseases at a nanoscopic resolution using whole corneal flat-mount beyond cell cultures.

Indexed as

AnimalsATP-Binding Cassette TransportersCorneaFluorescent Antibody TechniqueFluorescent DyesHistonesImage Processing, Computer-AssistedMiceMice, Inbred C57BLMicroscopy, ConfocalSingle Molecule ImagingStaining and LabelingTubulinVimentinAbcd3 protein, mouseATP-Binding Cassette TransportersFluorescent DyesHistonesTubulinVimentinVim protein, mouseCorneal endotheliumSingle-molecule localization microscopySuper-resolution optical microscopy

Identifiers

PMID33610603
PMCPMC8043998

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.