Evidence map›Paper›PMID 33609293›Full record

ArticleContact dermatitis2021

The expression pattern of N-acetyltransferase 1 in healthy human skin.

Cynthia C A van Amerongen, Duco Kramer, Hendri H Pas, Marie L A Schuttelaar

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Contact dermatitis, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 58% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Cynthia C A van AmerongenDepartment of Dermatology, University Medical Centre Groningen, University of Groningen, Groningen, The Netherlands.ORCID https://orcid.org/0000-0001-6091-6270
Duco KramerDepartment of Dermatology, University Medical Centre Groningen, University of Groningen, Groningen, The Netherlands.
Hendri H PasDepartment of Dermatology, University Medical Centre Groningen, University of Groningen, Groningen, The Netherlands.
Marie L A SchuttelaarDepartment of Dermatology, University Medical Centre Groningen, University of Groningen, Groningen, The Netherlands.ORCID https://orcid.org/0000-0002-0766-4382
University Medical Center Groningen · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundN-acetyltransferase 1 (NAT1) is an enzyme expressed among others in keratinocytes in human skin. NAT1 is important in the biotransformation of aromatic amines, an important example being p-phenylenediamine (PPD), a hair dye molecule. Unoxidized PPD penetrates the skin and is N-acetylated by NAT1.

objectivesTo investigate in detail the expression pattern of NAT1 in human skin. MATERIALS AND

methodsCryosections obtained from healthy human skin were stained for NAT1 and expression patterns were observed. NAT1 double stainings were performed with antibodies against different cellular organelles to determine expression patterns.

resultA speckled, granular expression of NAT1 was seen predominantly in the stratum basale. NAT1 was expressed in a cytoplasmic pattern, perinuclear, and in the nucleus. No co-localisation was seen with the selected cellular organelles. Local differences in NAT1 expression patterns were observed between donors and between different biopsies obtained from the same donor.

conclusionsNAT1 is expressed predominantly in the stratum basale and can be found in the cytoplasm, nucleus, and perinuclear in human skin. Further studies should be performed to investigate expression of NAT1 in a larger sample size.

Indexed as

AdolescentAdultArylamine N-AcetyltransferaseBiopsyFemaleHealthy VolunteersHumansIsoenzymesMaleMiddle AgedSkinStaining and LabelingArylamine N-AcetyltransferaseIsoenzymesN-acetyltransferase 1contact allergyhuman skinimmunofluorescenceN-acetyltransferase 1P-phenylenediamine

Identifiers

PMID33609293
PMCPMC8252542
OpenAlexW3133183361

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.