ArticleAMB Express2021
Efficient genome editing with CRISPR/Cas9 in Pleurotus ostreatus.
Article in AMB Express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 99 citations in OpenAlex.
- Molecular biology of Pleurotus mushrooms: genomic resources, genetic manipulation, and regulation mechanisms.Antonie van Leeuwenhoek · 2026Review
- Unlocking the Potential for Genetic Engineering of the Straw-Degrading MushroomJournal of fungi (Basel, Switzerland) · 2026Article
- Unraveling the Signaling Networks: How Exogenous Substances Mitigate Heat Stress in Edible Fungi.Journal of fungi (Basel, Switzerland) · 2026Review
- Molecular breeding alters mycelium material properties in the white-rot fungus Pleurotus ostreatus.Applied microbiology and biotechnology · 2026Article
- Targeted insertion of heterogenous DNA using Cas9-gRNA ribonucleoprotein-mediated gene editing inBioengineered · 2025Article
- Establishment of an antimetabolite-based transformation system for the wood-decaying basidiomyceteApplied and environmental microbiology · 2025Article
- Production of β-Glucans byInternational journal of molecular sciences · 2025Review
- Review on mushroom mycelium-based products and their production process: from upstream to downstream.Bioresources and bioprocessing · 2025Review
- Simultaneous gene editing of both nuclei in a dikaryotic strain of Ganoderma lucidum using Cas9-gRNA ribonucleoprotein.Journal of microbiology (Seoul, Korea) · 2025Article
- Towards engineering agaricomycete fungi for terpenoid production.Journal of industrial microbiology & biotechnology · 2024Review
- Trans-nuclei CRISPR/Cas9: safe approach for genome editing in the edible mushroom excluding foreign DNA sequences.Applied microbiology and biotechnology · 2024Article
- Effect of a Mating Type Gene Editing inJournal of fungi (Basel, Switzerland) · 2024Article
- Pleurotus ostreatus as a model mushroom in genetics, cell biology, and material sciences.Applied microbiology and biotechnology · 2024Review
- Utilization of CRISPR-Cas genome editing technology in filamentous fungi: function and advancement potentiality.Frontiers in microbiology · 2024Review
- The Cas9-gRNA ribonucleoprotein complex-mediated editing of pyrG in Ganoderma lucidum and unexpected insertion of contaminated DNA fragments.Scientific reports · 2023Article
- Effects of glutamate oxaloacetate transaminase on reactive oxygen species in Ganoderma lucidum.Applied microbiology and biotechnology · 2023Article
- A genetic tool to express long fungal biosynthetic genes.Fungal biology and biotechnology · 2023Article
- Application progress of CRISPR/Cas9 genome-editing technology in edible fungi.Frontiers in microbiology · 2023Review
- Preassembled Cas9 Ribonucleoprotein-Mediated Gene Deletion Identifies the Carbon Catabolite Repressor and Its Target Genes in Coprinopsis cinerea.Applied and environmental microbiology · 2022Article
- Multiplex gene precise editing and large DNA fragment deletion by the CRISPR-Cas9-TRAMA system in edible mushroom Cordyceps militaris.Microbial biotechnology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Pleurotus ostreatus is one of the most commercially produced edible mushrooms worldwide. Improved cultivated strains with more useful traits have been obtained using classical breeding, which is laborious and time-consuming. Here, we attempted efficient gene mutagenesis using plasmid-based CRISPR/Cas9 as the first step for non-genetically modified (non-GM) P. ostreatus generation. Plasmids harboring expression cassettes of Cas9 and different single guide RNAs targeting fcy1 and pyrG were individually transferred into fungal protoplasts of the PC9 strain, which generated some strains exhibiting resistance to 5-fluorocytosine and 5-fluoroorotic acid, respectively. Genomic PCR followed by sequencing revealed small insertions/deletions or insertion of a fragment from the plasmid at the target site in some of the drug-resistant strains. The results demonstrated efficient CRISPR/Cas9-assisted genome editing in P. ostreatus, which could contribute to the molecular breeding of non-GM cultivated strains in the future. Furthermore, a mutation in fcy1 via homology-directed repair using this CRISPR/Cas9 system was also efficiently introduced, which could be applied not only for precise gene disruption, but also for insertions leading to heterologous gene expression in this fungus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.