Evidence map›Paper›PMID 33608835›Full record

ArticleCell and tissue banking2022

Cryo-banking of human spermatozoa by aseptic cryoprotectants-free vitrification in liquid air: Positive effect of elevated warming temperature.

Maria Diaz-Jimenez, Mengying Wang, Wanxue Wang, Evgenia Isachenko, Gohar Rahimi, Pradeep Kumar, Peter Mallmann, Melanie von Brandenstein, Manuel Hidalgo, Vladimir Isachenko

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In one paragraph

Article in Cell and tissue banking, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Maria Diaz-Jimenez *Department of Obstetrics and Gynaecology, Medical Faculty, Cologne University, 50931, Cologne, Germany.
Mengying Wang *Department of Obstetrics and Gynaecology, Medical Faculty, Cologne University, 50931, Cologne, Germany.
Wanxue WangDepartment of Obstetrics and Gynaecology, Medical Faculty, Cologne University, 50931, Cologne, Germany.
Evgenia IsachenkoDepartment of Obstetrics and Gynaecology, Medical Faculty, Cologne University, 50931, Cologne, Germany.
Gohar RahimiDepartment of Obstetrics and Gynaecology, Medical Faculty, Cologne University, 50931, Cologne, Germany.
Pradeep KumarDepartment of Obstetrics and Gynaecology, Medical Faculty, Cologne University, 50931, Cologne, Germany.
Peter MallmannDepartment of Obstetrics and Gynaecology, Medical Faculty, Cologne University, 50931, Cologne, Germany.
Melanie von BrandensteinUniversity Clinic for Urology and Urological Oncology, Medical Faculty, Cologne University, 50931, Cologne, Germany.
Manuel HidalgoVeterinary Reproduction Group, Department of Animal Medicine Surgery, University of Cordoba, 14071, Cordoba, Spain.
Vladimir IsachenkoDepartment of Obstetrics and Gynaecology, Medical Faculty, Cologne University, 50931, Cologne, Germany. vladimir.isachenko@gmail.com.ORCID http://orcid.org/0000-0002-3674-543X
University of Cologne · DEUniversity of Córdoba · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cryoprotectant-free vitrification is a common method for spermatozoa cryopreservation by direct plunging into liquid nitrogen. However, the commercial liquid nitrogen could be potentially contaminated by microorganisms. Warming temperature plays an essential role for quality of human spermatozoa after vitrification. This study aimed to evaluate comparatively a quality spermatozoa after vitrification in liquid nitrogen and clean liquid air as well as with two warming rates: at 42 °C and 45 °C. After performing of routine swim-up of normozoospermia samples, spermatozoa from the same ejaculate were divided into two groups: vitrified in liquid nitrogen (LN) and sterile liquid air (LA). Spermatozoa of LN group were warmed at 42 °C, and spermatozoa of LA groups were divided and warmed at 42 °C (LA42) and 45 °C (LA45). Then spermatozoa motility, reactive oxygen species (ROS), mitochondrial membrane potential (MMP), reactive nitrogen species (RNS), and viability were assessed. It was no found significant differences in quality of spermatozoa from LN and LA groups in the motility, ROS, MMP, RNS rates after warming at 42 °C. A tendency to obtain better spermatozoa quality was found with using of warming by 42 °C in comparison with 45 °C. It was concluded that cryoprotectant-free vitrification by direct dropping of human spermatozoa into clean liquid air can be used as an alternative to cooling in liquid nitrogen. Warming of spermatozoa at 42 °C allows to preserve the spermatozoa physiological parameters.

Indexed as

Semen PreservationVitrificationCryopreservationCryoprotective AgentsHumansMaleSpermatozoaSperm MotilityTemperatureCryoprotective AgentsAseptic liquid airElevated warming temperatureHuman spermatozoaVitrification

Identifiers

PMID33608835
OpenAlexW3129494395

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.