Evidence map›Paper›PMID 33608751›Full record

ArticleAnalytical and bioanalytical chemistry2021

Highly sensitive protein detection using recombinant spores and lateral flow immunoassay.

Wen-Zhi Lin, I-Cheng Ma, Jun-Pei Wang, Ping-Chun Hsieh, Cheng-Che Liu, Shao-Yi Hou

Abstract read
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In one paragraph

Article in Analytical and bioanalytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Wen-Zhi LinInstitute of Preventive Medicine, National Defense Medical Center, Taipei, 11490, Taiwan.
I-Cheng MaGraduate Institute of Chemical Engineering, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, 10608, Taiwan.
Jun-Pei WangGraduate Institute of Chemical Engineering, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, 10608, Taiwan.
Ping-Chun HsiehGraduate Institute of Biochemical and Biomedical Engineering, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, 10608, Taiwan.
Cheng-Che LiuGraduate Institute of Physiology, National Defense Medical Center, Taipei, 11490, Taiwan.
Shao-Yi HouGraduate Institute of Biochemical and Biomedical Engineering, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, 10608, Taiwan. f10955@ntut.edu.tw.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lateral flow immunoassays (LFIs) can be used to detect intact bacteria or spores; when gold nanoparticles (AuNPs) are used as the signal reporters, the detection limits are very low. Spore-based surface display has been widely studied for enzyme immobilization and live-nontoxic oral vaccines. In this study, recombinant spores were used to improve the sensitivity of a LFI. We developed a test kit that combines streptavidin-displayed spores with a LFI assay for rapid protein detection. The recombinant spores served as a signal amplifier and AuNPs were used as the signal reporters. For detection of β-galactosidase, which was used as the model protein, the detection limit was about 10

Indexed as

Bacillus subtilisBiosensing TechniquesGoldGreen Fluorescent ProteinsImmunoassayLimit of DetectionMetal NanoparticlesProteinsSpores, BacterialStreptavidinenhanced green fluorescent proteinGoldGreen Fluorescent ProteinsProteinsStreptavidinGold nanoparticlesGreen fluorescent proteinLateral flow immunoassaysProtein detectionRecombinant spore

Identifiers

PMID33608751

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.