ArticleScientific reports2021
Timescale of hole closure during plasma membrane repair estimated by calcium imaging and numerical modeling.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- The Calcium-Calpain-ALIX Axis is the Major Link Between Gasdermin-D Membrane Pores and Terminal Membrane Damage in Pyroptosis.Research square · 2026Article
- An Overview of 3D Bioprinting Impact on Cell Viability: From Damage Assessment to Protection Solutions.Journal of functional biomaterials · 2025Review
- The invasion pore induced by Toxoplasma gondii.EMBO reports · 2025Article
- Cytotoxic T-Lymphocyte-Associated Protein 4 Fused to a Modified Fragment of IgG1 Reduces Muscle Fiber Damage in a Model of Duchenne Muscular Dystrophy by Attenuating Proinflammatory Gene Expression in Myeloid Lineage Cells.The American journal of pathology · 2025Article
- Shape of the membrane neck around a hole during plasma membrane repair.Biophysical journal · 2024Article
- Annexin A7 mediates lysosome repair independently of ESCRT-III.Frontiers in cell and developmental biology · 2023Article
- Article
- Evaluation of fluorescence-based viability stains in cells dissociated from scleractinian coral Pocillopora damicornis.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plasma membrane repair is essential for eukaryotic cell life and is triggered by the influx of calcium through membrane wounds. Repair consists of sequential steps, with closure of the membrane hole being the key event that allows the cell to recover, thus identifying the kinetics of hole closure as important for clarifying repair mechanisms and as a quantitative handle on repair efficiency. We implement calcium imaging in MCF7 breast carcinoma cells subject to laser damage, coupled with a model describing the spatio-temporal calcium distribution. The model identifies the time point of hole closure as the time of maximum calcium signal. Analysis of cell data estimates the closure time as: [Formula: see text] s and [Formula: see text] s using GCaMP6s-CAAX and GCaMP6s probes respectively. The timescale was confirmed by independent time-lapse imaging of a hole during sealing. Moreover, the analysis estimates the characteristic time scale of calcium removal, the penetration depth of the calcium wave and the diffusion coefficient. Probing of hole closure times emerges as a strong universal tool for quantification of plasma membrane repair.
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