ArticleNature communications2021
Ice-nucleating proteins are activated by low temperatures to control the structure of interfacial water.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 104 citations in OpenAlex.
- Membrane vesicles associated antimicrobial peptides from PGPRPlant signaling & behavior · 2026Article
- Whole testicular organ and seminal cryopreservation in the malarial mosquito Anopheles gambiae (Diptera: Culicidae).Journal of medical entomology · 2026Article
- Impact of Perfluorinated Organic Acids on Bacterial Ice Nucleators.The journal of physical chemistry. B · 2026Article
- Genomic Characterization ofPlants (Basel, Switzerland) · 2026Article
- Genetic Surfaceome E. coli Reprogramming Enables Selective Water Oxidation.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Microbial Enhancement of Plant Tolerance to Waterlogging: Mechanisms and Interplay with Biological Control of Pathogens.International journal of molecular sciences · 2025Review
- Chromatic forecasting hydrogels for anti-icing applications.Nature communications · 2025Article
- Structural adaptability and surface activity of peptides derived from tardigrade proteins.Protein science : a publication of the Protein Society · 2024Article
- High-speed cryo-microscopy reveals that ice-nucleating proteins ofScience advances · 2024Article
- Effect of Aggregation and Molecular Size on the Ice Nucleation Efficiency of Proteins.Environmental science & technology · 2024Article
- Article
- Ice nucleation proteins self-assemble into large fibres to trigger freezing at near 0 °C.eLife · 2023Article
- Ice nucleation proteins self-assemble into large fibres to trigger freezing at near 0 °C.bioRxiv : the preprint server for biology · 2023Article
- Crowding alters F-actin secondary structure and hydration.Communications biology · 2023Article
- A general overview of the multifactorial adaptation to cold: biochemical mechanisms and strategies.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Review
- Coping with the cold: unveiling cryoprotectants, molecular signaling pathways, and strategies for cold stress resilience.Frontiers in plant science · 2023Review
- Shape-dependent gold nanoparticle interactions with a model cell membrane.Biointerphases · 2022Article
- Effect of diffusion kinetics on the ice nucleation temperature distribution.Scientific reports · 2022Article
- Identification of Candidate Ice Nucleation Activity (INA) Genes inJournal of fungi (Basel, Switzerland) · 2022Article
- Water-organizing motif continuity is critical for potent ice nucleation protein activity.Nature communications · 2022Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ice-nucleation active (INA) bacteria can promote the growth of ice more effectively than any other known material. Using specialized ice-nucleating proteins (INPs), they obtain nutrients from plants by inducing frost damage and, when airborne in the atmosphere, they drive ice nucleation within clouds, which may affect global precipitation patterns. Despite their evident environmental importance, the molecular mechanisms behind INP-induced freezing have remained largely elusive. We investigate the structural basis for the interactions between water and the ice-nucleating protein InaZ from the INA bacterium Pseudomonas syringae. Using vibrational sum-frequency generation (SFG) and two-dimensional infrared spectroscopy, we demonstrate that the ice-active repeats of InaZ adopt a β-helical structure in solution and at water surfaces. In this configuration, interaction between INPs and water molecules imposes structural ordering on the adjacent water network. The observed order of water increases as the interface is cooled to temperatures close to the melting point of water. Experimental SFG data combined with molecular-dynamics simulations and spectral calculations show that InaZ reorients at lower temperatures. This reorientation can enhance water interactions, and thereby the effectiveness of ice nucleation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.