ArticleGenome biology2021
FlsnRNA-seq: protoplasting-free full-length single-nucleus RNA profiling in plants.
Article in Genome biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 100 citations in OpenAlex.
- Review
- A systematic benchmark of bioinformatics methods for single-cell and spatial RNA-seq nanopore long reads data.NAR genomics and bioinformatics · 2026Article
- Advances in single-cell and spatial multi-omics for plant development and stress responses.Plant diversity · 2026Review
- Single-Nucleus Transcriptome Analysis Provides New Insights Into B Chromosome Elimination in Sorghum.Plant biotechnology journal · 2026Article
- Cell-type specific early perception of nine phytohormones revealed by single-nucleus transcriptomics in Arabidopsis.Nature communications · 2026Article
- Nanopore direct RNA sequencing and the epitranscriptome: Advances in mapping native RNA landscapes.iMeta · 2026Review
- Integrating scRNA-seq and snRNA-seq with spatial transcriptomics to unlock the xylem puzzle.Genome biology · 2026Article
- Review
- Identification of Phloem Cell Transcriptome via Single-Cell RNA-Sequencing.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Pangenomics and single-cell transcriptomics uncover the genetic basis of continuous bearing trait in grapevine.Horticulture research · 2025Article
- Single-cell transcriptome atlas unveils transcriptional regulation networks of banana root tips in response toHorticulture research · 2025Article
- Single-nucleus multi-omics reveals the impact of drought stress on the development of soybean endosperm.Plant communications · 2025Article
- Single-cell transcriptome sequencing reveals the sphingolipid metabolism pathway plays an important role in leaf senescence in tobacco.BMC plant biology · 2025Article
- Exploring the untapped potential of single-cell and spatial omics in plant biology.The New phytologist · 2025Review
- Integration of single-nuclei transcriptome and bulk RNA-seq to unravel the role of AhWRKY70 in regulating stem cell development in Arachis hypogaea L.Plant biotechnology journal · 2025Article
- Comparative single-nucleus RNA-seq analysis revealed localized and cell type-specific pathways governing root-microbiome interactions.Nature communications · 2025Article
- Orthologous marker groups reveal broad cell identity conservation across plant single-cell transcriptomes.Nature communications · 2025Article
- An improved nuclei isolation protocol from leaf tissue for single-cell transcriptomics.PloS one · 2025Article
- Single-cell transcriptomic and cell‑type‑specific regulatory networks in Polima temperature-sensitive cytoplasmic male sterility of Brassica napus L.BMC plant biology · 2024Article
- Direct RNA sequencing in plants: Practical applications and future perspectives.Plant communications · 2024Review
2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
The broad application of single-cell RNA profiling in plants has been hindered by the prerequisite of protoplasting that requires digesting the cell walls from different types of plant tissues. Here, we present a protoplasting-free approach, flsnRNA-seq, for large-scale full-length RNA profiling at a single-nucleus level in plants using isolated nuclei. Combined with 10x Genomics and Nanopore long-read sequencing, we validate the robustness of this approach in Arabidopsis root cells and the developing endosperm. Sequencing results demonstrate that it allows for uncovering alternative splicing and polyadenylation-related RNA isoform information at the single-cell level, which facilitates characterizing cell identities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.