ArticlePloS one2021
Direct evaluation of self-quenching behavior of fluorophores at high concentrations using an evanescent field.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Fluorescence quenching-based methods for pharmaceutical analysis: a comprehensive review of principles, probes, and applications.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2026Review
- Reversible Nucleolar Complex Coacervation by Short Cationic Peptides.Journal of the American Chemical Society · 2026Article
- A Sustainable Spectrofluorimetric Quenching Approach for Determination of Pentoxifylline or Donepezil HCl via Complexation With Erythrosine B: Application to Tablets and Human Plasma.Luminescence : the journal of biological and chemical luminescence · 2026Article
- QuantGUV: Quantifying Encapsulation Efficiency of Small Molecules in Giant Unilamellar Vesicles.ACS applied materials & interfaces · 2026Article
- Fluorescence bioimaging of drug nanocarriers based on Förster resonance energy transfer, aggregation-induced emission and aggregation-caused quenching.Acta pharmaceutica Sinica. B · 2026Review
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- Longitudinal Assessment of Fluorescence Stability Shows Fluorescence Intensity Decreases Over Time: Implications for Fluorescence Microscopy Studies.bioRxiv : the preprint server for biology · 2025Article
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- A collagen IV fluorophore knock-in toolkit reveals trimer diversity in C. elegans basement membranes.The Journal of cell biology · 2025Article
- Individual ZnO-Ag Hybrid Nanorods for Synergistic Fluorescence Enhancement Towards Highly Sensitive and Miniaturized Biodetection.Nanomaterials (Basel, Switzerland) · 2025Article
- Modulating Adjuvant Release Kinetics From Scaffold Vaccines to Tune Adaptive Immune Responses.Advanced healthcare materials · 2025Article
- Mapping drug distribution using CT imaging following direct tissue injection in ex vivo liver: informing clinical implementation.CVIR oncology · 2025Article
- Oligo(ethylene glycol) Methacrylate Copolymer-Modified Liposomes for Temperature-Responsive Drug Delivery System.Molecules (Basel, Switzerland) · 2024Article
- Photopolymerization of Chlorpromazine-Loaded Gelatin Methacryloyl Hydrogels: Characterization and Antimicrobial Applications.Gels (Basel, Switzerland) · 2024Article
- Chemo- and regio-selective differential modification of native cysteines on an antibodyChemical science · 2024Article
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Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The quantum yield of a fluorophore is reduced when two or more identical fluorophores are in close proximity to each other. The study of protein folding or particle aggregation is can be done based on this above-mentioned phenomenon-called self-quenching. However, it is challenging to characterize the self-quenching of a fluorophore at high concentrations because of the inner filter effect, which involves depletion of excitation light and re-absorption of emission light. Herein, a novel method to directly evaluate the self-quenching behavior of fluorophores was developed. The evanescent field from an objective-type total internal reflection fluorescence (TIRF) microscope was used to reduce the path length of the excitation and emission light to ~100 nm, thereby supressing the inner filter effect. Fluorescence intensities of sulforhodamine B, fluorescein isothiocyanate (FITC), and calcein solutions with concentrations ranging from 1 μM to 50 mM were directly measured to evaluate the concentration required for 1000-fold degree of self-quenching and to examine the different mechanisms through which the fluorophores undergo self-quenching.
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