Evidence map›Paper›PMID 33606221›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Cryopreservation and Transplantation of Spermatogonial Stem Cells.

Zoran Marinović, Jelena Lujić, Qian Li, Yoshiko Iwasaki, Béla Urbányi, Goro Yoshizaki, Ákos Horváth

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
3.5field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

Zoran MarinovićDepartment of Aquaculture, Szent István University, Gödöllő, Páter Károly u. 1., Hungary.
Jelena LujićDepartment of Biomedical Sciences, Center for Reproductive Genomics, Cornell University, Ithaca, NY, USA.
Qian LiNational Engineering Center for Biochip at Shanghai, Shanghai Biochip Limited Corporation, Shanghai, People's Republic of China.
Yoshiko IwasakiDepartment of Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo, Japan.
Béla UrbányiDepartment of Aquaculture, Szent István University, Gödöllő, Páter Károly u. 1., Hungary.
Goro YoshizakiDepartment of Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo, Japan.
Ákos HorváthDepartment of Aquaculture, Szent István University, Gödöllő, Páter Károly u. 1., Hungary. Horvath.Akos@szie.hu.
Magyar Agrár- és Élettudományi Egyetem · HUTokyo University of Marine Science and Technology · JPCornell University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cryopreservation as a method that enables long-term storage of biological material has long been used for the conservation of valuable zebrafish genetic resources. However, currently, only spermatozoa of zebrafish can be successfully cryopreserved, while protocols for cryopreservation of eggs and embryos have not yet been fully developed. Transplantation of germline stem cells (GSCs) has risen as a favorable method that can bypass the current problem in cryopreservation of female genetic resources and can lead to reconstitution of fish species and lines through surrogate production. Here, we describe essential steps needed for the cryopreservation of spermatogonial stem cells (SSCs) and their utilization in the conservation of zebrafish genetic resources through SSC transplantation and surrogate production.

Indexed as

Adult Germline Stem CellsAnimalsCryopreservationCryoprotective AgentsMaleSpermatogoniaSpermatozoaTestisTransplantationZebrafishCryoprotective AgentsBiobankingConservationFreezingMicroinjectionSurrogate production

Identifiers

PMID33606221
OpenAlexW3132814053

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.