Evidence map›Paper›PMID 33606214›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Generation of Transgenic Fluorescent Reporter Lines for Studying Hematopoietic Development in the Mouse.

Jeffrey Barminko, Andrei M Vacaru, Margaret H Baron

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.7field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Jeffrey BarminkoDepartment of Medicine, The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Andrei M VacaruDepartment of Medicine, The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Margaret H BaronDepartments of Medicine, Cell, Developmental, and Regenerative Biology, and Oncological Sciences, The Tisch Cancer Institute, The Black Family Stem Cell Institute, and The Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA. margaret.baron@mssm.edu.
Icahn School of Medicine at Mount Sinai · US

Funding

Regulation of Erythropoiesis by the VDR Nuclear Receptor Transcription FactorR01DK102945 · NIDDK · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI BARON, MARGARET H · 2015 to 2022
$4.0M
NIDDK NIH HHS R01 DK102945
6 · The paper itself

Abstract

Hematopoiesis in the mouse and other mammals occurs in several waves and arises from distinct anatomic sites. Transgenic mice expressing fluorescent reporter proteins at various points in the hematopoietic hierarchy, from hematopoietic stem cell to more restricted progenitors to each of the final differentiated cell types, have provided valuable tools for tagging, tracking, and isolating these cells. In this chapter, we discuss general considerations in designing a transgene, survey available fluorescent probes, and describe methods for confirming and analyzing transgene expression in the hematopoietic tissues of the embryo, fetus, and postnatal/adult animal.

Indexed as

AnimalsCell DifferentiationEmbryo, MammalianFemaleFetusGenes, ReporterHematopoiesisHematopoietic Stem CellsLuminescent ProteinsMaleMiceMice, TransgenicStem CellsTransgenesLuminescent ProteinsFluorescent reporterGreen fluorescent proteinHematopoiesisKnock-inTransgenic mice

Identifiers

PMID33606214
OpenAlexW3130079559

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.