Evidence map›Paper›PMID 33606204›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Analysis of Gene Expression Using lacZ Reporter Mouse Lines.

Michael Simon Krämer, Robert Feil, Hannes Schmidt

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. A novelFrontiers in physiology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Michael Simon KrämerInterfaculty Institute of Biochemistry, Eberhard Karls University Tübingen, Tübingen, Germany.
Robert FeilInterfaculty Institute of Biochemistry, Eberhard Karls University Tübingen, Tübingen, Germany.
Hannes SchmidtInterfaculty Institute of Biochemistry, Eberhard Karls University Tübingen, Tübingen, Germany. hannes.schmidt@uni-tuebingen.de.
University of Tübingen · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reporter mice transgenically expressing the bacterial (E. coli) lacZ gene encoding β-galactosidase (β-gal, EC 3.2.1.23) are a versatile and extensively used tool to study gene expression and cell lineage patterns. Enzymatic activity of the β-gal reporter can be effectively visualized at cellular resolution either histochemically using 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) or by immunofluorescent detection using a β-gal-specific antibody. Here, we summarize protocols for the localization of β-gal expressing cells in whole embryos or organs as well as in histological tissue sections of lacZ reporter mice and discuss their limitations and common pitfalls.

Indexed as

AnimalsCell LineageEmbryo, MammalianEscherichia coliGalactosidesGene ExpressionGenes, ReporterIndolesLac OperonMiceStaining and Labeling5-bromo-4-chloro-3-indolyl beta-galactosideGalactosidesIndolesGene expressionImmunofluorescence staininglacZReporter miceX-galβ-galactosidase

Identifiers

PMID33606204
OpenAlexW3132352267

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.