ArticleMicrobial biotechnology2021
High-efficiency production of bisabolene from waste cooking oil by metabolically engineered Yarrowia lipolytica.
Article in Microbial biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Utilization, metabolic regulation and applications of hydrophobic substrates inSynthetic and systems biotechnology · 2026Review
- Engineering ofACS omega · 2026Article
- Production of the terpenoid-based biofuel α-bisabolene by metabolically engineered Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2025Article
- Enhanced vitamin A production by engineering transporters, ATP and precursor supply.Biodesign research · 2025Article
- Review
- Production of the Sesquiterpene Bisabolene From One- and Two-Carbon Compounds in Engineered Methanosarcina acetivorans.Microbial biotechnology · 2025Article
- Strategies for the Transformation of Waste Cooking Oils into High-Value Products: A Critical Review.Polymers · 2025Review
- CRISPR-Mediated rDNA Integration and Fluorescence Screening for Pathway Optimization inChem & bio engineering · 2024Article
- Engineering Yeast Peroxisomes for α-Bisabolene Production from Sole Methanol with the Aid of Proteomic Analysis.JACS Au · 2024Article
- Engineering strategies for enhanced 1', 4'-trans-ABA diol production by Botrytis cinerea.Microbial cell factories · 2024Article
- Biosynthesis Progress of High-Energy-Density Liquid Fuels Derived from Terpenes.Microorganisms · 2024Review
- Progress in the Metabolic Engineering ofBiodesign research · 2024Review
- Larvicidal proficiency of volatile compounds present inFrontiers in plant science · 2023Article
- Over-expression of α-bisabolene by metabolic engineering of Yarrowia lipolytica employing a golden gate DNA assembly toolbox.Biotechnology notes (Amsterdam, Netherlands) · 2023Article
- Engineering ofMetabolic engineering communications · 2022Review
- Enhanced production of amyrin in Yarrowia lipolytica using a combinatorial protein and metabolic engineering approach.Microbial cell factories · 2022Article
- Recent advances in construction and regulation of yeast cell factories.World journal of microbiology & biotechnology · 2022Review
- Toward improved terpenoids biosynthesis: strategies to enhance the capabilities of cell factories.Bioresources and bioprocessing · 2022Review
- EngineeringFrontiers in bioengineering and biotechnology · 2022Article
- High-efficiency production of bisabolene from waste cooking oil by metabolically engineered Yarrowia lipolytica.Microbial biotechnology · 2021Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The natural plant product bisabolene serves as a precursor for the production of a wide range of industrially relevant chemicals. However, the low abundance of bisabolene in plants renders its isolation from plant sources non-economically viable. Therefore, creation of microbial cell factories for bisabolene production supported by synthetic biology and metabolic engineering strategies presents a more competitive and environmentally sustainable method for industrial production of bisabolene. In this proof-of-principle study, for the first time, we engineered the oleaginous yeast Yarrowia lipolytica to produce α-bisabolene, β-bisabolene and γ-bisabolene through heterologous expression of the α-bisabolene synthase from Abies grandis, the β-bisabolene synthase gene from Zingiber officinale and the γ-bisabolene synthase gene from Helianthus annuus respectively. Subsequently, two metabolic engineering approaches, including overexpression of the endogenous mevalonate pathway genes and introduction of heterologous multidrug efflux transporters, were employed in order to improve bisabolene production. Furthermore, the fermentation conditions were optimized to maximize bisabolene production by the engineered Y. lipolytica strains from glucose. Finally, we explored the potential of the engineered Y. lipolytica strains for bisabolene production from the waste cooking oil. To our knowledge, this is the first report of bisabolene production in Y. lipolytica using metabolic engineering strategies. These findings provide valuable insights into the engineering of Y. lipolytica for a higher-level production of bisabolene and its utilization in converting waste cooking oil into various industrially valuable products.
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