ArticleeLife2021
Redox controls RecA protein activity via reversible oxidation of its methionine residues.
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Implications of bacterial exposure to reactive oxygen and chlorine species.Infection and immunity · 2026Review
- Beyond Resistance: Tolerance and Resilience of Bacteria to Photodynamic and Oxidative Stress.International journal of molecular sciences · 2025Review
- Repair of oxidized methionine residues in the chaperone Spy maintains periplasmic proteostasis under chlorite stress in Escherichia coli.PLoS biology · 2025Article
- From the Gut to the Brain: Transcriptomic Insights into Neonatal MeningitisPathogens (Basel, Switzerland) · 2025Article
- Collective peroxide detoxification determines microbial mutation rate plasticity in E. coli.PLoS biology · 2024Article
- Bacillus subtilis improves antioxidant capacity and optimizes inflammatory state in broilers.Animal bioscience · 2024Article
- Generation and Repair of Postreplication Gaps in Escherichia coli.Microbiology and molecular biology reviews : MMBR · 2023Review
- A Year at the Forefront of Bacterial Defense Systems Against Neutrophilic Oxidants.Biology open · 2023Article
- Methionine oxidation under anaerobic conditions in Escherichia coli.Molecular microbiology · 2022Article
- A Novel Small RNA, DsrO, in Deinococcus radiodurans Promotes Methionine Sulfoxide Reductase (Applied and environmental microbiology · 2022Article
- Escherichia coli induces DNA repair enzymes to protect itself from low-grade hydrogen peroxide stress.Molecular microbiology · 2022Article
- Thiol Reductases inAntioxidants (Basel, Switzerland) · 2022Review
- Comprehensive Temporal Protein Dynamics during Postirradiation Recovery inOxidative medicine and cellular longevity · 2022Article
- Mechanism of O-Atom Transfer from Nitrite: Nitric Oxide Release at Copper(II).Inorganic chemistry · 2021Article
- Design and comparative characterization of RecA variants.Scientific reports · 2021Article
- Redox controls RecA protein activity via reversible oxidation of its methionine residues.eLife · 2021Article
- Bacterial Response to Oxidative Stress and RNA Oxidation.Frontiers in genetics · 2021Review
- Methionine Redox Homeostasis in Protein Quality Control.Frontiers in molecular biosciences · 2021Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 4 countries.
Funding
Abstract
Reactive oxygen species (ROS) cause damage to DNA and proteins. Here, we report that the RecA recombinase is itself oxidized by ROS. Genetic and biochemical analyses revealed that oxidation of RecA altered its DNA repair and DNA recombination activities. Mass spectrometry analysis showed that exposure to ROS converted four out of nine Met residues of RecA to methionine sulfoxide. Mimicking oxidation of Met35 by changing it for Gln caused complete loss of function, whereas mimicking oxidation of Met164 resulted in constitutive SOS activation and loss of recombination activity. Yet, all ROS-induced alterations of RecA activity were suppressed by methionine sulfoxide reductases MsrA and MsrB. These findings indicate that under oxidative stress MsrA/B is needed for RecA homeostasis control. The implication is that, besides damaging DNA structure directly, ROS prevent repair of DNA damage by hampering RecA activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.