Evidence map›Paper›PMID 33604582›Full record

ArticleSTAR protocols2021

Biochemical detection of capsid in the nucleus during HIV-1 infection.

Anastasia Selyutina, Felipe Diaz-Griffero

Abstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Mechanism-guided engineering of a minimal biological particle for genome editing.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
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  3. Review
  4. Article
  5. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Anastasia SelyutinaDepartment of Microbiology and Immunology, Einstein, Bronx, NY 10461, USA.
Felipe Diaz-GrifferoDepartment of Microbiology and Immunology, Einstein, Bronx, NY 10461, USA.

Funding

Role of Cellular Factors in Retroviral Uncoating and Synthesis of Viral DNAR01AI087390 · NIAID · ALBERT EINSTEIN COLLEGE OF MEDICINE, INC · PI DIAZ-GRIFFERO, FELIPE · 2010 to 2024
$6.8M
NIAID NIH HHS R01 AI087390
6 · The paper itself

Abstract

To understand the role of the HIV-1 capsid in viral replication, we developed a protocol to biochemically track capsid in the nucleus during infection. To this end, we separated HIV-1-infected cells into nuclear and cytosolic fractions. Fractions were analyzed by western blotting for HIV-1 capsid content as well as for nuclear and cytosolic markers to assess the bona fide origin of the fractions. This protocol can be applied in both cycling and non-cycling human cells. For complete details on the use and execution of this protocol, please refer to Selyutina et al. (2020a).

Indexed as

Cell NucleusCytosolHIV InfectionsVirus ReplicationA549 CellsAnimalsCapsidDogsHEK293 CellsHeLa CellsHIV-1HumansCell biologyMolecular biologyProtein biochemistry

Identifiers

PMID33604582
PMCPMC7873705

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.