ArticleScience (New York, N.Y.)2021
Cholangiocyte organoids can repair bile ducts after transplantation in the human liver.
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Article in Science (New York, N.Y.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 197 papers, 2 of them syntheses that pooled it.
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197citing papers in PubMed, 2 pooled it
30.2field-weighted citation impact, top 1% of its field
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Who cites it
197 citing papers in PubMed, 2 syntheses or guidelines pooled it, 356 citations in OpenAlex.
- Bile Duct Replacement in Hepatobiliary Surgery: A Systematic Review.World journal of surgery · 2025Pooled it
- Biliary Viability Assessment and Treatment Options of Biliary Injury During Normothermic Liver Perfusion-A Systematic Review.Transplant international : official journal of the European Society for Organ Transplantation · 2022Pooled it
- Systematic production of human kidney organoids for transplantation in porcine kidneys during ex vivo machine perfusion.Nature biomedical engineering · 2026Article
- JAG1 c.1615C > T mutation impairs bile duct regeneration but not differentiation in hepatic organoids derived from a patient with Alagille syndrome.Stem cells translational medicine · 2026Article
- Farnesoid X receptor activates STAT3 to promote fatty acid oxidation and suppress inflammatory macrophage responses in cholestasis.The Journal of biological chemistry · 2026Article
- The making of cells and organs in xenogeneic animal bioreactor.Signal transduction and targeted therapy · 2026Review
- Liver Organoids: From Disease Modelling to Regenerative Medicine.Cell proliferation · 2026Review
- The RNA helicase domain of NAT10 promotes the biogenesis of hypomodified ribosomes to enhance cancer cell proliferation.Nature communications · 2026Article
- From Preservation to Repair: A Systematic Review of Therapeutic Organ Rehabilitation During Normothermic Ex Vivo Machine Perfusion.Transplantation direct · 2026Review
- Synthetic developmental engineering of human liver organogenesis.Development (Cambridge, England) · 2026Review
- Organoids: From Bench to Bedside Applications.MedComm · 2026Review
- Organoids: generation strategies, applications, and future challenges.Stem cell research & therapy · 2026Review
- Stem Cell Therapies for Gastrointestinal and Liver Diseases: Translational Barriers, Clinical Heterogeneity, and Future Directions.Biomedicines · 2026Review
- Automated and scalable expansion of human liver organoids for translational applications.Journal of translational medicine · 2026Article
- Human amniotic epithelial stem cells promote cholangiocyte regeneration via Notch signaling pathway in bile duct injury.Chinese medical journal · 2026Article
- Hepatitis E virus replication is maintained in proliferative cells within the intestinal crypt.Science advances · 2026Article
- Mechanochemical regulation of organoid morphogenesis: Integrated signaling circuits in engineered microenvironments.Materials today. Bio · 2026Review
- Three-dimensional bioprinted hiHeps hepatorganoids with enhanced hepatic functions for the treatment of liver failure and promotion of liver regeneration.Bioactive materials · 2026Article
- [Research progress on the mechanisms and optimization strategies of mesenchymal stem cell-derived exosomes in the treatment of liver diseases].Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2026Review
- Organoid Technology as a Promising Frontier in Organ Transplantation and Regenerative Medicine.International journal of stem cells · 2026Review
137 more citing papers are in PubMed but not listed here.
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Authors and funding
40 authors at 8 institutions in 5 countries.
Fotios SampaziotisWellcome-MRC Cambridge Stem Cell Institute, Cambridge, Cambridgeshire, UK. fs347@cam.ac.uk lv225@cam.ac.uk.ORCID https://orcid.org/0000-0003-0812-7586
Daniele MuraroWellcome-MRC Cambridge Stem Cell Institute, Cambridge, Cambridgeshire, UK.ORCID https://orcid.org/0000-0001-9601-237X
Olivia C TysoeWellcome-MRC Cambridge Stem Cell Institute, Cambridge, Cambridgeshire, UK.ORCID https://orcid.org/0000-0002-1061-1484
Stephen SawiakDepartment of Clinical Neurosciences, University of Cambridge, Cambridge, Cambridgeshire, UK.ORCID https://orcid.org/0000-0003-4210-9816
Timothy E BeachDepartment of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, Cambridgeshire, UK.
Edmund M GodfreyDepartment of Radiology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, Cambridgeshire, UK.
Sara S UpponiDepartment of Radiology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, Cambridgeshire, UK.
Teresa BreviniWellcome-MRC Cambridge Stem Cell Institute, Cambridge, Cambridgeshire, UK.ORCID https://orcid.org/0000-0002-3581-5379
Brandon T WesleyWellcome-MRC Cambridge Stem Cell Institute, Cambridge, Cambridgeshire, UK.ORCID https://orcid.org/0000-0003-0530-329X
Jose Garcia-BernardoWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridgeshire, UK.ORCID https://orcid.org/0000-0003-3626-5433
Krishnaa MahbubaniDepartment of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, Cambridgeshire, UK.ORCID https://orcid.org/0000-0002-1327-2334
Giovanni CanuWellcome-MRC Cambridge Stem Cell Institute, Cambridge, Cambridgeshire, UK.ORCID https://orcid.org/0000-0002-3349-4479
Richard GieseckImmunopathogenesis Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Natalie L BerntsenNorwegian PSC Research Center, Department of Transplantation Medicine, Division of Surgery, Inflammatory Diseases and Transplantation, Oslo University Hospital, Rikshospitalet, Oslo, Norway.ORCID https://orcid.org/0000-0002-5949-8910
Victoria L MulcahyDepartment of Medicine, University of Cambridge, Cambridge, Cambridgeshire, UK.ORCID https://orcid.org/0000-0001-7091-4789
Keziah CrickDepartment of Surgery, Cambridge University Hospitals NHS Foundation Trust, Cambridge, Cambridgeshire, UK.
Corrina FearDepartment of Surgery, Cambridge University Hospitals NHS Foundation Trust, Cambridge, Cambridgeshire, UK.
Sharayne RobinsonDepartment of Surgery, Cambridge University Hospitals NHS Foundation Trust, Cambridge, Cambridgeshire, UK.ORCID https://orcid.org/0000-0001-6819-3433
Lisa SwiftDepartment of Surgery, Cambridge University Hospitals NHS Foundation Trust, Cambridge, Cambridgeshire, UK.ORCID https://orcid.org/0000-0001-9044-688X
Laure GambardellaWellcome-MRC Cambridge Stem Cell Institute, Cambridge, Cambridgeshire, UK.ORCID https://orcid.org/0000-0001-5771-1565
Johannes BargehrWellcome-MRC Cambridge Stem Cell Institute, Cambridge, Cambridgeshire, UK.ORCID https://orcid.org/0000-0002-9304-3573
Daniel OrtmannWellcome-MRC Cambridge Stem Cell Institute, Cambridge, Cambridgeshire, UK.
Stephanie E BrownWellcome-MRC Cambridge Stem Cell Institute, Cambridge, Cambridgeshire, UK.
Anna OsnatoWellcome-MRC Cambridge Stem Cell Institute, Cambridge, Cambridgeshire, UK.ORCID https://orcid.org/0000-0001-5241-1512
Michael P MurphyMRC Mitochondrial Biology Unit, University of Cambridge, Cambridge, Cambridgeshire, UK.ORCID https://orcid.org/0000-0003-1115-9618
Gareth CorbettCambridge University Hospitals NHS Foundation Trust, Cambridge, Cambridgeshire, UK.
William T H GelsonDepartment of Medicine, University of Cambridge, Cambridge, Cambridgeshire, UK.
George F MellsDepartment of Medicine, University of Cambridge, Cambridge, Cambridgeshire, UK.
Peter HumphreysWellcome-MRC Cambridge Stem Cell Institute, Cambridge, Cambridgeshire, UK.
Susan E DaviesDepartment of Histopathology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, Cambridgeshire, UK.
Irum AminDepartment of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, Cambridgeshire, UK.
Paul GibbsDepartment of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, Cambridgeshire, UK.ORCID https://orcid.org/0000-0003-2193-5377
Sanjay SinhaWellcome-MRC Cambridge Stem Cell Institute, Cambridge, Cambridgeshire, UK.ORCID https://orcid.org/0000-0001-5900-1209
Sarah A TeichmannWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridgeshire, UK.ORCID https://orcid.org/0000-0002-6294-6366
Andrew J ButlerDepartment of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, Cambridgeshire, UK.
Teik Choon SeeDepartment of Radiology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, Cambridgeshire, UK.
Espen MelumNorwegian PSC Research Center, Department of Transplantation Medicine, Division of Surgery, Inflammatory Diseases and Transplantation, Oslo University Hospital, Rikshospitalet, Oslo, Norway.ORCID https://orcid.org/0000-0001-6903-6878
Christopher J E WatsonDepartment of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, Cambridgeshire, UK.
Kourosh Saeb-Parsy *Department of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, Cambridgeshire, UK.ORCID https://orcid.org/0000-0002-0633-3696
Ludovic Vallier *Wellcome-MRC Cambridge Stem Cell Institute, Cambridge, Cambridgeshire, UK. fs347@cam.ac.uk lv225@cam.ac.uk.ORCID https://orcid.org/0000-0002-3848-2602
Wellcome/MRC Cambridge Stem Cell Institute · GBUniversity of Cambridge · GBCambridge University Hospitals NHS Foundation Trust · GBOslo University Hospital · NOMRC Mitochondrial Biology Unit · GBNational Institutes of Health · USNHS Blood and Transplant · GBWellcome Sanger Institute · GB
Funding
British Heart Foundation FS/18/46/33663British Heart Foundation PG/17/24/32886Medical Research Council G0701448Medical Research Council MC_PC_17230Medical Research Council MC_UU_12012/5National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/N001540/1National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/T2T0419Wellcome Trust 203151
Abstract
Organoid technology holds great promise for regenerative medicine but has not yet been applied to humans. We address this challenge using cholangiocyte organoids in the context of cholangiopathies, which represent a key reason for liver transplantation. Using single-cell RNA sequencing, we show that primary human cholangiocytes display transcriptional diversity that is lost in organoid culture. However, cholangiocyte organoids remain plastic and resume their in vivo signatures when transplanted back in the biliary tree. We then utilize a model of cell engraftment in human livers undergoing ex vivo normothermic perfusion to demonstrate that this property allows extrahepatic organoids to repair human intrahepatic ducts after transplantation. Our results provide proof of principle that cholangiocyte organoids can be used to repair human biliary epithelium.
Indexed as
Cell- and Tissue-Based TherapyAnimalsBileBile Duct DiseasesBile DuctsBile Ducts, IntrahepaticCommon Bile DuctEpithelial CellsGallbladderGene Expression RegulationHumansLiverLiver TransplantationMesenchymal Stem Cell TransplantationMiceOrganoids
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.