Evidence map›Paper›PMID 33602319›Full record

ArticleVeterinary research2021

Rhomboid protein 2 of Eimeria maxima provided partial protection against infection by homologous species.

Yufeng Chen, Di Tian, Lixin Xu, Ruofeng Yan, Xiangrui Li, Muhammad Ali A Shah, Xiaokai Song

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Prokaryotic Expression ofInternational journal of molecular sciences · 2022
    Article
  2. Protective efficacy induced byFrontiers in veterinary science · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yufeng Chen *MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
Di Tian *MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
Lixin XuMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
Ruofeng YanMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
Xiangrui LiMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
Muhammad Ali A ShahDepartment of Pathobiology, PMAS Arid Agriculture University, Rawalpindi, 41000, Pakistan.
Xiaokai SongMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China. songxiaokai@njau.edu.cn.ORCID http://orcid.org/0000-0003-0102-0938

Funding

National Key Research and Development Program of China 2017YFD0500400National Natural Science Foundation of China 31661143017National Natural Science Foundation of China 31672545National Natural Science Foundation of China 31972705
6 · The paper itself

Abstract

Rhomboid-like proteases (ROMs) are considered as new candidate antigens for developing new-generation vaccines due to their important role involved in the invasion of apicomplexan protozoa. In prior works, we obtained a ROM2 sequence of Eimeria maxima (EmROM2). This study was conducted to evaluate the immunogenicity and protective efficacy of EmROM2 recombinant protein (rEmROM2) and EmROM2 DNA (pVAX1-EmROM2) against infection by Eimeria maxima (E. maxima). Firstly, Western blot assay was conducted to analyze the immunogenicity of rEmROM2. The result showed that rEmROM2 was recognized by chicken anti-E. maxima serum. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot assay revealed apparent transcription and expression of EmROM2 at the injection site. qRT-PCR (quantitative real-time PCR), flow cytometry and indirect ELISA indicated that vaccination with rEmROM2 or EmROM2 DNA significantly upregulated the transcription level of cytokines (IFN-γ, IL-2, IL-4, IL-10, IL-17, TGF-β and TNF SF15), the proportion of CD8

Indexed as

AnimalsChickensCloning, MolecularCoccidiosisEimeriaGene Expression RegulationImmunization, SecondaryImmunoglobulin GPeptide HydrolasesProtozoan ProteinsProtozoan VaccinesRatsRats, Sprague-DawleyRecombinant ProteinsImmunoglobulin GPeptide HydrolasesProtozoan ProteinsProtozoan VaccinesRecombinant ProteinsEimeria maximaImmunogenicityProtective efficacyRhomboid-like protein 2

Identifiers

PMID33602319
PMCPMC7893758

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.