ArticleCurrent protocols2021
In Vitro Cultivation of the Syphilis Spirochete Treponema pallidum.
Article in Current protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 46 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 65 citations in OpenAlex.
- Neurosyphilis-Induced Psychosis in Europe: A Systematic Review of Case Reports.Pathogens (Basel, Switzerland) · 2024Pooled it
- A New Era in Treponema pallidum Cultivation: Current Advances and Future Directions.Sexually transmitted diseases · 2026Review
- Formal description ofInternational journal of systematic and evolutionary microbiology · 2026Article
- Human placental trophoblasts support sustainedbioRxiv : the preprint server for biology · 2026Article
- TheJornal brasileiro de doencas sexualmente transmissiveis : DST · 2026Article
- Review
- Proteome Analysis of SevenJournal of proteome research · 2025Article
- In vitro analysis of seven syphilis-causing Treponema pallidum strains revealed inherent growth rate differences.Scientific reports · 2025Article
- Identification of antibodies induced by immunization with the syphilis vaccine candidate Tp0751.Vaccine · 2025Article
- Tp40: a new potential prognostic and diagnostic marker for syphilis.Microbiology spectrum · 2025Article
- Establishment of the Nichols strain as the type strain ofInternational journal of systematic and evolutionary microbiology · 2025Review
- Treponema denticola major surface protein (Msp): a key player in periodontal pathogenicity and immune evasion.Archives of microbiology · 2025Review
- Development and utilization ofmBio · 2025Article
- Time-course transcriptomics reveals the impact ofFrontiers in microbiology · 2025Article
- Exploitation of Biodiversity in Bioeconomy: Examples, Opportunities, and Challenges.Advances in biochemical engineering/biotechnology · 2025Review
- Article
- Antibodies directed against extracellular loops of FadL orthologs disrupt outer membrane integrity and neutralize infectivity ofFrontiers in immunology · 2025Article
- Clinical Presentation of Early Syphilis and Genomic Sequences of Treponema pallidum Strains in Patient Specimens and Isolates Obtained by Rabbit Inoculation.The Journal of infectious diseases · 2024Article
- The Significance of the Cell-Mediated Host Immune Response in Syphilis.Microorganisms · 2024Review
- Immunodominant extracellular loops of Treponema pallidum FadL outer membrane proteins elicit antibodies with opsonic and growth-inhibitory activities.PLoS pathogens · 2024Article
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
For over a century, investigation of Treponema pallidum subsp. pallidum, the spiral-shaped bacterium that causes syphilis, was hindered by an inability to culture the organism in vitro. A recent breakthrough has enabled continuous in vitro growth of this organism in co-culture with mammalian tissue culture cells. This article contains the protocols needed to culture T. pallidum in the standard laboratory environment. In addition, protocols for growing and maintaining the required tissue culture cells, for generating isogenic strains by limiting dilution, and for quantitating T. pallidum by darkfield microscopy are included. © 2021 Wiley Periodicals LLC. Basic Protocol 1: In vitro cultivation of Treponema pallidum Basic Protocol 2: Generation of isogenic strains Support Protocol 1: Alternate harvest procedure Support Protocol 2: Culture of Sf1Ep cells Support Protocol 3: Assessment of T. pallidum number and viability.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.