Evidence map›Paper›PMID 33597641›Full record

ArticleScientific reports2021

Isolation and characterization of novel primary cells from the human distal outflow pathway.

Uttio Roy Chowdhury, Cindy K Bahler, Cheryl R Hann, Bradley H Holman, Michael P Fautsch

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Uttio Roy ChowdhuryDepartment of Ophthalmology, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905, USA. roychowdhury.uttio@mayo.edu.
Cindy K BahlerDepartment of Ophthalmology, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905, USA.
Cheryl R HannDepartment of Ophthalmology, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905, USA.
Bradley H HolmanDepartment of Ophthalmology, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905, USA.
Michael P FautschDepartment of Ophthalmology, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905, USA.
Mayo Clinic · US

Funding

Intraocular pressure regulation via ATP-sensitive potassium channelsR01EY021727 · NEI · MAYO CLINIC ROCHESTER · PI FAUTSCH, MICHAEL P. · 2011 to 2021
$4.1M
NEI NIH HHS R01 EY021727NIH HHS EY21727
6 · The paper itself

Abstract

Ocular hypertension occurs due to increased resistance to aqueous humor removal through the conventional outflow pathway. Unlike the proximal region of the conventional outflow pathway, the distal region has not been well studied, mostly due to lack of model systems. Here we describe isolation and characterization of human primary vascular distal outflow pathway (VDOP) cells from the distal region of the conventional outflow pathway. Tissue from the distal region was isolated from human corneo-scleral rims, digested with collagenase type I (100 U/ml) and placed on gelatin coated plates to allow cellular growth in Dulbecco's Modified Eagle's Medium (low glucose) containing fetal bovine serum and antibiotic/antimycotic. VDOP cells showed consistent proliferation for up to 7 passages, retained endothelial-like nature of the parent tissues and showed a unique marker phenotype of Lectin

Indexed as

ActinsAdultAgedAged, 80 and overAqueous HumorChildChild, PreschoolCytoskeletal ProteinsEndotheliumEyeEye ProteinsFemaleGlaucomaGlycoproteinsHumansIntraocular PressureActinsCytoskeletal ProteinsEye ProteinsGlycoproteinsMyocilin

Identifiers

PMID33597641
PMCPMC7890058
OpenAlexW3131983468

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.