ArticleScience advances2021
Microrheology reveals simultaneous cell-mediated matrix stiffening and fluidization that underlie breast cancer invasion.
Article in Science advances, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Matrix stress relaxation drives glioblastoma cell response in viscoelastic biomaterials.Science advances · 2026Article
- Microscale Mechanical Cues in Hydrogels: Engineering Strategies to Modulate Cell Fates in Three Dimensions.Cell biomaterials · 2026Article
- Assessing the micromechanical architecture of joint tissues with speckle rheological microscopy.Nature communications · 2026Article
- Engineering Complexity: Advances in 3D Breast Cancer Models for Precision Oncology.Advanced healthcare materials · 2026Review
- A biofabricated 3D cancer-stroma tumor microenvironment model.Biofabrication · 2025Article
- Tumor Organoids Grown in Mixed-Composition Hydrogels Recapitulate the Plasticity of Pancreatic Cancers.Gels (Basel, Switzerland) · 2025Article
- Unveiling Matrix Metalloproteinase 13's Dynamic Role in Breast Cancer: A Link to Physical Changes and Prognostic Modulation.International journal of molecular sciences · 2025Review
- Probabilistic analysis of spatial viscoelastic cues in 3D cell culture using magnetic microrheometry.Biophysical journal · 2025Article
- Speckle fluctuations reveal dynamics of microparticles in fibrin scaffolds in a model of bacterial infection.npj biological physics and mechanics · 2025Article
- Small molecular weight epigenetic inhibitors modulate the extracellular matrix during pancreatic acinar ductal metaplasia.Biochemical and biophysical research communications · 2024Article
- Embedded 3D Bioprinting of Collagen Inks into Microgel Baths to Control Hydrogel Microstructure and Cell Spreading.Advanced healthcare materials · 2024Article
- Viscoelasticity in 3D Cell Culture and Regenerative Medicine: Measurement Techniques and Biological Relevance.ACS materials Au · 2024Review
- Laser speckle rheological microscopy reveals wideband viscoelastic spectra of biological tissues.Science advances · 2024Article
- Engineering Heterogeneous Tumor Models for Biomedical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Standardization of organoid culture in cancer research.Cancer medicine · 2023Review
- Local response and emerging nonlinear elastic length scale in biopolymer matrices.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- 3D bioprinting of dynamic hydrogel bioinks enabled by small molecule modulators.Science advances · 2023Article
- Recent advances in optical elastography and emerging opportunities in the basic sciences and translational medicine [Invited].Biomedical optics express · 2023Review
- Spatial collagen stiffening promotes collective breast cancer cell invasion by reinforcing extracellular matrix alignment.Oncogene · 2022Article
- Cell-matrix reciprocity in 3D culture models with nonlinear elasticity.Bioactive materials · 2022Review
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Authors and funding
7 authors.
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Abstract
Living tissues embody a unique class of hybrid materials in which active and thermal forces are inextricably linked. Mechanical characterization of tissues demands descriptors that respect this hybrid nature. In this work, we develop a microrheology-based force spectrum analysis (FSA) technique to dissect the active and passive fluctuations of the extracellular matrix (ECM) in three-dimensional (3D) cell culture models. In two different stromal models and a 3D breast cancer spheroid model, our FSA reveals emergent hybrid dynamics that involve both high-frequency stress stiffening and low-frequency fluidization of the ECM. We show that this is a general consequence of nonlinear coupling between active forces and the frequency-dependent viscoelasticity of stress-stiffening networks. In 3D breast cancer spheroids, this dual active stiffening and fluidization is tightly connected with invasion. Our results suggest a mechanism whereby breast cancer cells reconcile the seemingly contradictory requirements for both tension and malleability in the ECM during invasion.
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