ArticlePlant methods2021
Heritable gene editing using FT mobile guide RNAs and DNA viruses.
Article in Plant methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 74 citations in OpenAlex.
- Transforming Plant Viruses into Vectors for Next-Generation Agriculture-A Review.Current microbiology · 2026Review
- Genetic transformation of forage crops: comparative barriers, evidence, and emerging strategies.Plant cell reports · 2026Review
- Transgene-free plant genome editing via viral delivery of miniature CRISPR-Cas12f.Functional & integrative genomics · 2026Article
- Virus-induced genome editing enables functional genomics across diverse plant species.Molecules and cells · 2026Review
- Challenges and Opportunities with CRISPR-Based Genome Editing in Legume Crops.Functional & integrative genomics · 2026Review
- Beyond genes: CRISPR/Cas9 for chromosomal engineering in polyploids.Frontiers in genome editing · 2026Article
- Florigen and Florigen-Like Genes Regulate Temperature-Responsive Flowering in Tomato.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- CRISPR/Cas system-mediated transgene-free or DNA-free genome editing in plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Virus-induced gene editing free from tissue culture.Nature plants · 2025Review
- Virus-induced systemic and heritable gene editing in pepper (Capsicum annuum L.).The Plant journal : for cell and molecular biology · 2025Article
- Virus-Induced Genome Editing (VIGE): One Step Away from an Agricultural Revolution.International journal of molecular sciences · 2025Review
- Heritable virus-induced germline editing in tomato.The Plant journal : for cell and molecular biology · 2025Article
- Current Advancement and Future Prospects in Simplified Transformation-Based Plant Genome Editing.Plants (Basel, Switzerland) · 2025Review
- CRISPR/Cas9-mediated editing of eukaryotic elongation factor 1B gamma (eEF1Bγ) reduces Tobacco etch virus accumulation in Nicotiana benthamiana.Plant cell reports · 2025Article
- Enhancing virus-mediated genome editing for cultivated tomato through low temperature.Plant cell reports · 2025Article
- Enhancing CRISPR-Cas-based gene targeting in tomato using a dominant-negativeHorticulture research · 2025Article
- Tissue culture-independent approaches to revolutionizing plant transformation and gene editing.Horticulture research · 2025Article
- Heritable Tissue-Culture-Free Gene Editing in Nicotiana benthamiana through Viral Delivery of SpCas9 and sgRNA.Plant & cell physiology · 2024Article
- Trans-complementation of the viral movement protein mediates efficient expression of large target genes via a tobacco mosaic virus vector.Plant biotechnology journal · 2024Article
- A comprehensive review of in planta stable transformation strategies.Plant methods · 2024Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe virus-induced genome editing (VIGE) system can be used to quickly identify gene functions and generate knock-out libraries as an alternative to the virus-induced gene silencing (VIGS). Although plant virus-mediated VIGE has been shown to have great application prospects, edited genes cannot be transferred to the next generations using this system, as viruses cannot enter into shoot apical meristem (SAM) in plants.
resultsWe developed a novel cotton leaf crumple virus (CLCrV)-mediated VIGE system designed to target BRI1, GL2, PDS genes, and GUS transgene in A. thaliana by transforming Cas9 overexpression (Cas9-OE) A. thaliana. Given the deficiency of the VIGE system, ProYao::Cas9 and Pro35S::Cas9 A. thaliana were transformed by fusing 102 bp FT mRNAs with sgRNAs so as to explore the function of Flowering Locus T (FT) gene in delivering sgRNAs into SAM, thus avoiding tissue culture and stably acquiring heritable mutant offspring. Our results showed that sgRNAs fused with FT mRNA at the 5' end (FT strategy) effectively enabled gene editing in infected plants and allowed the acquisition of mutations heritable by the next generation, with an efficiency of 4.35-8.79%. In addition, gene-edited offspring by FT-sgRNAs did not contain any components of the CLCrV genome.
conclusionsFT strategy can be used to acquire heritable mutant offspring avoiding tissue culture and stable transformation based on the CLCrV-mediated VIGE system in A. thaliana.
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