ArticleAnalytical chemistry2021
Robust Microflow LC-MS/MS for Proteome Analysis: 38 000 Runs and Counting.
Article in Analytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 72 citations in OpenAlex.
- Biomolecular profiling for noninvasive health monitoring.Nature biotechnology · 2026Review
- Factor VIII restores bone parameters and modulates muscle proteo-metabolome in Factor VIII knockout male mice.Bone research · 2026Article
- High-Performance Proteomics Using Nano-, Capillary-, and Microflow Chromatographic Separations.Journal of proteome research · 2025Article
- CySP3-96 Enables Scalable, Streamlined, and Low-Cost Sample Preparation for Cysteine Chemoproteomic Applications.Molecular & cellular proteomics : MCP · 2025Article
- New Approaches To Identify Urine and Hair Adulteration Attempts in Forensic Toxicology: A Proof-of-Concept Study Using a Proteomics Approach Based on Liquid Chromatography-Mass Spectrometry (LC-MS).Analytical chemistry · 2025Article
- Enhancing Tandem MS Sensitivity and Peptide Identification via Ion Preaccumulation in an Orbitrap Mass Spectrometer.Journal of proteome research · 2025Article
- StageTip: a little giant unveiling the potential of mass spectrometry-based proteomics.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Review
- Leveraging the potential of 1.0-mm i.d. columns in UHPLC-HRMS-based untargeted metabolomics.Analytical and bioanalytical chemistry · 2025Article
- Methodological Aspects of μLC-MS/MS for Wide-Scale Proteomic Analysis of Anthracycline-Induced Cardiomyopathy.ACS omega · 2025Article
- Proteomic Profiling Towards a Better Understanding of Genetic Based Muscular Diseases: The Current Picture and a Look to the Future.Biomolecules · 2025Review
- The Circulating Proteome─Technological Developments, Current Challenges, and Future Trends.Journal of proteome research · 2024Article
- Considerations for Method Development and Method Translation in Capillary Liquid Chromatography: A Tutorial.Journal of chromatography open · 2024Article
- The Future of Proteomics is Up in the Air: Can Ion Mobility Replace Liquid Chromatography for High Throughput Proteomics?Journal of proteome research · 2024Review
- Improved Data Acquisition Settings on Q Exactive HF-X and Fusion Lumos Tribrid Orbitrap-Based Mass Spectrometers for Proteomic Analysis of Limited Samples.Journal of proteome research · 2024Article
- SpectiCal:Journal of proteome research · 2024Article
- High-Throughput Microbore LC-MS Lipidomics to Investigate APOE Phenotypes.Analytical chemistry · 2024Article
- Illuminating phenotypic drug responses of sarcoma cells to kinase inhibitors by phosphoproteomics.Molecular systems biology · 2024Article
- A region-resolved proteomic map of the human brain enabled by high-throughput proteomics.The EMBO journal · 2023Article
- Vacuum Insulated Probe Heated Electrospray Ionization Source Enhances Microflow Rate Chromatography Signals in the Bruker timsTOF Mass Spectrometer.Journal of proteome research · 2023Article
- Vacuum Insulated Probe Heated ElectroSpray Ionization source (VIP-HESI) enhances micro flow rate chromatography signals in the Bruker timsTOF mass spectrometer.bioRxiv : the preprint server for biology · 2023Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Microflow liquid chromatography tandem mass spectrometry (μLC-MS/MS) is becoming a viable alternative to nanoflow LC-MS/MS for the analysis of proteomes. We have recently demonstrated the potential of such a system operating with a 1 mm i.d. × 150 mm column and at a flow rate of 50 μL/min for high-throughput applications. On the basis of the analysis of ∼38 000 samples measured on two instruments over the past two years, we now show that the approach is extremely robust. Up to 1500 analyses were performed within one month, and >14 000 samples could be analyzed on a single column without loss of chromatographic performance. Samples included proteomes of cell lines, tissues, and human body fluids, which were analyzed with or without prior peptide fractionation or stable isotope labeling. We show that the μLC-MS/MS system is capable of measuring 2600 proteins from undepleted human plasma and ∼5000 proteins from crude human urine in 1 day, demonstrating its potential for in-depth as well as high-throughput clinical application.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.