Evidence map›Paper›PMID 33595397›Full record

ReviewCritical reviews in clinical laboratory sciences2021

Molecular diagnostic assays for COVID-19: an overview.

Parham Habibzadeh, Mohammad Mofatteh, Mohammad Silawi, Saeid Ghavami, Mohammad Ali Faghihi

Abstract readReview
In one paragraph

Review in Critical reviews in clinical laboratory sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Changes in microRNA expression associated with preeclampsia: a systematic review.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2025
    Pooled it
  2. Pooled it
  3. Article
  4. Article
  5. Review
  6. RT-qPCR Testing and Performance Metrics in the COVID-19 Era.International journal of molecular sciences · 2024
    Review
  7. Spotted fever diagnosis using molecular methods.Revista da Sociedade Brasileira de Medicina Tropical · 2024
    Review
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  9. Article
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  11. Article
  12. Review
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  18. Observational
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Parham HabibzadehPersian BayanGene Research and Training Center, Shiraz University of Medical Sciences, Shiraz, Iran.ORCID 0000-0002-2086-0425
Mohammad MofattehSir William Dunn School of Pathology, University of Oxford, Oxford, UK.
Mohammad SilawiPersian BayanGene Research and Training Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Saeid GhavamiMax Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada.ORCID 0000-0001-5948-508X
Mohammad Ali FaghihiPersian BayanGene Research and Training Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The coronavirus disease 2019 (COVID-19) pandemic has highlighted the cardinal importance of rapid and accurate diagnostic assays. Since the early days of the outbreak, researchers with different scientific backgrounds across the globe have tried to fulfill the urgent need for such assays, with many assays having been approved and with others still undergoing clinical validation. Molecular diagnostic assays are a major group of tests used to diagnose COVID-19. Currently, the detection of SARS-CoV-2 RNA by reverse transcription polymerase chain reaction (RT-PCR) is the most widely used method. Other diagnostic molecular methods, including CRISPR-based assays, isothermal nucleic acid amplification methods, digital PCR, microarray assays, and next generation sequencing (NGS), are promising alternatives. In this review, we summarize the technical and clinical applications of the different COVID-19 molecular diagnostic assays and suggest directions for the implementation of such technologies in future infectious disease outbreaks.

Indexed as

Molecular Diagnostic TechniquesNucleic Acid Amplification TechniquesCOVID-19COVID-19 TestingHumansSARS-CoV-2CoronavirusCOVID-19molecular diagnostic techniquesnucleic acid amplification techniquesSARS-CoV-2

Identifiers

PMID33595397
PMCPMC7898297

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.