ArticleAnalytical chemistry2021
Analysis of Fixed and Live Single Cells Using Optical Photothermal Infrared with Concomitant Raman Spectroscopy.
Article in Analytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Demystifying Experimental Parameters in Optical Photothermal Imaging in Aqueous Media.ACS omega · 2026Article
- Intrinsic OPTIR imaging and spectroscopy enable label-free monitoring of viral infection in living cells.Analytical and bioanalytical chemistry · 2026Article
- Leveraging image processing techniques to visualize sub-cellular domains in optical photothermal infrared imaging.The Analyst · 2026Article
- Nanoscale-Sensitive VSFG Microscopy for Identifying Breast Tumor in FFPE Samples.Chemical & biomedical imaging · 2026Article
- IR Map of the Human Cell.Analytical chemistry · 2026Article
- Biological and Biomedical Applications of Optical Photothermal Infrared Spectroscopy (O-PTIR).Applied spectroscopy · 2026Review
- The CHI3L1 protein plays role as a protecting factor against autophagy in glioblastoma cells.Apoptosis : an international journal on programmed cell death · 2026Article
- Investigating Spectral Biomarker Candidates for Migratory Potential in Cancer Cells Using Micro-FTIR and O‑PTIR Spectroscopy.ACS measurement science au · 2026Article
- Label-free protein-structure-sensitive live-cell microscopy for patient-specific assessment of myeloma therapy.Nature biomedical engineering · 2026Article
- Feeling the vibes: Vibrational spectroscopic imaging of biomolecular assemblies in their natural environment.Applied physics reviews · 2025Article
- Mode-Specific Coherent Interference of Vibrational Sum-Frequency Generation Imaging: An Approach to Differentiate Lung Tumors through Collagen Interfibrillar Distances.Journal of the American Chemical Society · 2025Article
- Vibrational spectroscopy unveils distinct cell cycle features of cancer stem cells in melanoma.Scientific reports · 2025Article
- Optical Photothermal Infrared Imaging Using Metabolic Probes in Biological Systems.Analytical chemistry · 2025Article
- INSPIRE: Single-beam probed complementary vibrational bioimaging.Science advances · 2024Article
- Identification of tauopathy-associated lipid signatures in Alzheimer's disease mouse brain using label-free chemical imaging.Communications biology · 2024Article
- A tutorial on optical photothermal infrared (O-PTIR) microscopy.APL photonics · 2024Article
- Structural Mapping of Protein Aggregates in Live Cells Modeling Huntington's Disease.Angewandte Chemie (International ed. in English) · 2024Article
- An optical photothermal infrared investigation of lymph nodal metastases of oral squamous cell carcinoma.Scientific reports · 2024Article
- Mid-infrared Photothermal Imaging: Instrument and Life Science Applications.Analytical chemistry · 2024Review
- Oleic acid differentially affects lipid droplet storage ofChemical communications (Cambridge, England) · 2024Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
This paper reports the first use of a novel completely optically based photothermal method (O-PTIR) for obtaining infrared spectra of both fixed and living cells using a quantum cascade laser (QCL) and optical parametric oscillator (OPO) laser as excitation sources, thus enabling all biologically relevant vibrations to be analyzed at submicron spatial resolution. In addition, infrared data acquisition is combined with concomitant Raman spectra from exactly the same excitation location, meaning the full vibrational profile of the cell can be obtained. The pancreatic cancer cell line MIA PaCa-2 and the breast cancer cell line MDA-MB-231 are used as model cells to demonstrate the capabilities of the new instrumentation. These combined modalities can be used to analyze subcellular structures in both fixed and, more importantly, live cells under aqueous conditions. We show that the protein secondary structure and lipid-rich bodies can be identified on the submicron scale.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.