Evidence map›Paper›PMID 33594376›Full record

ArticlemedRxiv : the preprint server for health sciences2021

Antigen-based multiplex strategies to discriminate SARS-CoV-2 natural and vaccine induced immunity from seasonal human coronavirus humoral responses.

Eric D Laing, Spencer L Sterling, Stephanie A Richard, Nusrat J Epsi, Si'Ana Coggins, Emily C Samuels, Shreshta Phogat, Lianying Yan, Nicole Moreno, Christian L Coles and 19 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in medRxiv : the preprint server for health sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 36 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

29 authors at 5 institutions in 1 country.

Eric D LaingDepartment of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Spencer L SterlingDepartment of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Stephanie A RichardHenry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD USA.
Nusrat J EpsiHenry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD USA.
Si'Ana CogginsDepartment of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Emily C SamuelsDepartment of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Shreshta PhogatDepartment of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Lianying YanDepartment of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Nicole MorenoHenry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD USA.
Christian L ColesHenry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD USA.
Matthew DrewProtein Expression Laboratory, National Cancer Institute RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Jennifer MehalkoProtein Expression Laboratory, National Cancer Institute RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Caroline E EnglishHenry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD USA.
Scott MerrittHenry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD USA.
Katrin MendeHenry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD USA.
Vincent J MunsterLaboratory of Virology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.
Emmie de WitLaboratory of Virology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.
Kevin K ChungDepartment of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Eugene V MillarHenry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD USA.
David R TribbleInfectious Disease Clinical Research Program, Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Mark P SimonsDepartment of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Simon D PollettHenry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD USA.
Brian K AganHenry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD USA.
Dominic EspositoProtein Expression Laboratory, National Cancer Institute RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Charlotte LanteriHenry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD USA.
G Travis CliftonBrooke Army Medical Center, JBSA Fort Sam Houston, TX, USA.
Edward MitreDepartment of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Timothy H BurgessHenry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD USA.
Christopher C BroderDepartment of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Henry M. Jackson Foundation · USUniformed Services University of the Health Sciences · USProtein Express (United States) · USNational Institutes of Health · USBrooke Army Medical Center · US

Funding

DATA CENTER FOR EPIDEMIOLOGIC INVESTIGATIONS OF HIVN01AI005072 · NIAID · NEW ENGLAND RESEARCH INSTITUTES, INC. · PI MCKINLAY, SONJA M · 1990 to 1994
–
CCR NIH HHS HHSN261200800001CNCI NIH HHS HHSN261200800001ENIAID NIH HHS Y01 AI005072
6 · The paper itself

Abstract

Sensitive and specific SARS-CoV-2 antibody assays remain critical for community and hospital-based SARS-CoV-2 sero-surveillance. With the rollout of SARS-CoV-2 vaccines, such assays must be able to distinguish vaccine from natural immunity to SARS-CoV-2 and related human coronaviruses. Here, we developed and implemented multiplex microsphere-based immunoassay strategies for COVD-19 antibody studies that incorporates spike protein trimers of SARS-CoV-2 and the endemic seasonal human coronaviruses (HCoV), enabling high throughout measurement of pre-existing cross-reactive antibodies. We varied SARS-CoV-2 antigen compositions within the multiplex assay, allowing direct comparisons of the effects of spike protein, receptor-binding domain protein (RBD) and nucleocapsid protein (NP) based SARS-CoV-2 antibody detection. Multiplex immunoassay performance characteristics are antigen-dependent, and sensitivities and specificities range 92-99% and 94-100%, respectively, for human subject samples collected as early as 7-10 days from symptom onset. SARS-CoV-2 spike and RBD had a strong correlative relationship for the detection of IgG. Correlation between detectable IgG reactive with spike and NP also had strong relationship, however, several PCR-positive and spike IgG-positive serum samples were NP IgG-negative. This spike and NP multiplex immunoassay has the potential to be useful for differentiation between vaccination and natural infection induced antibody responses. We also assessed the induction of

Identifiers

PMID33594376
PMCPMC7885935
OpenAlexW3131646327

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.