Evidence map›Paper›PMID 33594126›Full record

ArticleScientific reports2021

Polarimetric imaging microscopy for advanced inspection of vegetal tissues.

Albert Van Eeckhout, Enrique Garcia-Caurel, Teresa Garnatje, Juan Carlos Escalera, Mercè Durfort, Josep Vidal, José J Gil, Juan Campos, Angel Lizana

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
5.1field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 37 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Albert Van EeckhoutGrup D'Òptica, Physics Department, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain. albert.vaneeckhout@uab.cat.
Enrique Garcia-CaurelLPICM, CNRS, Ecole Polytechnique, Institut Polytechnique de Paris, 91120, Palaiseau, France.
Teresa GarnatjeBotanical Institute of Barcelona (IBB, CSIC-ICUB), 08038, Barcelona, Spain.
Juan Carlos EscaleraGrup D'Òptica, Physics Department, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain.
Mercè DurfortDepartament de Biologia Cellular, Fisiologia & Immunologia. Facultat de Biologia, Universitat de Barcelona, 08028, Barcelona, Spain.
Josep VidalGrup D'Òptica, Physics Department, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain.
José J GilDepartment of Applied Physics, University of Zaragoza, Pedro Cerbuna 12, 50009, Zaragoza, Spain.
Juan CamposGrup D'Òptica, Physics Department, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain.
Angel LizanaGrup D'Òptica, Physics Department, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain.
Universitat Autònoma de Barcelona · ESÉcole Polytechnique · FRInstitut Botànic de Barcelona · ESUniversidad de Zaragoza · ESUniversitat de Barcelona · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Optical microscopy techniques for plant inspection benefit from the fact that at least one of the multiple properties of light (intensity, phase, wavelength, polarization) may be modified by vegetal tissues. Paradoxically, polarimetric microscopy although being a mature technique in biophotonics, is not so commonly used in botany. Importantly, only specific polarimetric observables, as birefringence or dichroism, have some presence in botany studies, and other relevant metrics, as those based on depolarization, are underused. We present a versatile method, based on a representative selection of polarimetric observables, to obtain and to analyse images of plants which bring significant information about their structure and/or the spatial organization of their constituents (cells, organelles, among other structures). We provide a thorough analysis of polarimetric microscopy images of sections of plant leaves which are compared with those obtained by other commonly used microscopy techniques in plant biology. Our results show the interest of polarimetric microscopy for plant inspection, as it is non-destructive technique, highly competitive in economical and time consumption, and providing advantages compared to standard non-polarizing techniques.

Identifiers

PMID33594126
PMCPMC7887219
OpenAlexW3130922751

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.