Evidence map›Paper›PMID 33594001›Full record

ArticlemSystems2021

Comparative Fungal Community Analyses Using Metatranscriptomics and Internal Transcribed Spacer Amplicon Sequencing from Norway Spruce.

Andreas N Schneider, John Sundh, Görel Sundström, Kerstin Richau, Nicolas Delhomme, Manfred Grabherr, Vaughan Hurry, Nathaniel R Street

Open access · goldAbstract read
In one paragraph

Article in mSystems, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. Review
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  10. Review
  11. Metatranscriptomics captures dynamic shifts in mycorrhizal coordination in boreal forests.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Andreas N SchneiderUmeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, Sweden.ORCID https://orcid.org/0000-0001-5431-955X
John SundhDepartment of Biochemistry and Biophysics, National Bioinformatics Infrastructure Sweden, Science for Life Laboratory, Stockholm University, Solna, Sweden.
Görel SundströmUmeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish Agricultural University, Umeå, Sweden.
Kerstin RichauUmeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, Sweden.
Nicolas DelhommeUmeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish Agricultural University, Umeå, Sweden.
Manfred GrabherrDepartment of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Vaughan HurryUmeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish Agricultural University, Umeå, Sweden.
Nathaniel R StreetUmeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, Sweden nathaniel.street@umu.se.ORCID https://orcid.org/0000-0001-6031-005X
Umeå Plant Science Centre · SEStockholm University · SEUppsala University · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The health, growth, and fitness of boreal forest trees are impacted and improved by their associated microbiomes. Microbial gene expression and functional activity can be assayed with RNA sequencing (RNA-Seq) data from host samples. In contrast, phylogenetic marker gene amplicon sequencing data are used to assess taxonomic composition and community structure of the microbiome. Few studies have considered how much of this structural and taxonomic information is included in transcriptomic data from matched samples. Here, we described fungal communities using both host-derived RNA-Seq and fungal ITS1 DNA amplicon sequencing to compare the outcomes between the methods. We used a panel of root and needle samples from the coniferous tree species

Indexed as

ectomycorrhizafungiITS amplicon sequencingmetatranscriptomicsNorway sprucenutrient enrichmentphyllospherephyllosphere-inhabiting microbestree roots

Identifiers

PMID33594001
PMCPMC8573963
OpenAlexW3133034498

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.