ArticleProceedings of the National Academy of Sciences of the United States of America2021
Nile Red fluorescence spectroscopy reports early physicochemical changes in myelin with high sensitivity.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 89 citations in OpenAlex.
- Seeing the Chemistry of Biomolecular Condensates: In Situ Mapping of Composition and Water Content.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Experimental induction of myelin damage in post-mortem human brain slice cultures.Acta neuropathologica communications · 2026Article
- Molecular Origins of the Optical Response of Nile Red in Model Aerosol Aqueous Ionic Environments.The journal of physical chemistry. A · 2026Article
- Molecular Extraction and Release of an Ionic Liquid Microdroplet With Dynamic Tunability Under Optical Trapping.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- Fast Hyperspectral and Super-Resolved Mapping of Lipid Membrane Polarity with Single-Molecule Sensitivity.Chemical & biomedical imaging · 2026Article
- Biallelic GLTP mutations cause nonsyndromic epidermal differentiation disorder via disrupted epidermal glucosylceramide transport.The Journal of clinical investigation · 2026Article
- Advances in multiscale myelin imaging: from classical histology to functional insights.Frontiers in cellular neuroscience · 2026Review
- Single molecule spectrum dynamics imaging with 3D target-locking tracking.Nature communications · 2025Article
- Comparative Analysis of Histological Tools for Myelin.Neuroscience bulletin · 2025Review
- Three-Phase Emulsions during Cross-Coupling Reactions in Water.The Journal of organic chemistry · 2025Article
- A focus on the normal-appearing white and gray matter within the multiple sclerosis brain: a link to smoldering progression.Acta neuropathologica · 2025Review
- Tuning Hyaluronic Acid Microstructures by Engineered Amphiphilicity: From Dynamically Cross-Linked Gels to Multilayered Nanoparticles.ACS applied materials & interfaces · 2025Article
- Multiple sclerosis: what have we learned and can we still learn from electron microscopy.Cellular and molecular life sciences : CMLS · 2025Review
- Quantitation of the physicochemical properties of myelin using Nile Red fluorescence spectroscopy.Journal of neurochemistry · 2025Article
- Nile Red Fluorescence: Where's the Twist?The journal of physical chemistry. B · 2024Article
- Oil-in-Water Emulsions Probed Using Fluorescence Multivariate-Curve-Resolution Spectroscopy.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Three-Dimensional Imaging of Bioinspired Lipidic Mesophases Using Multicolored Light-Emitting Carbon Nanodots.The journal of physical chemistry letters · 2024Article
- Ratiometric fluorescence nanoscopy and lifetime imaging of novel Nile Red analogs for analysis of membrane packing in living cells.Scientific reports · 2024Article
- Micro-diffusely abnormal white matter: An early multiple sclerosis lesion phase with intensified myelin blistering.Annals of clinical and translational neurology · 2024Article
- Evidence of Heritability in Prebiotically Realistic Membrane-Bound Systems.Life (Basel, Switzerland) · 2024Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
The molecular composition of myelin membranes determines their structure and function. Even minute changes to the biochemical balance can have profound consequences for axonal conduction and the synchronicity of neural networks. Hypothesizing that the earliest indication of myelin injury involves changes in the composition and/or polarity of its constituent lipids, we developed a sensitive spectroscopic technique for defining the chemical polarity of myelin lipids in fixed frozen tissue sections from rodent and human. The method uses a simple staining procedure involving the lipophilic dye Nile Red, whose fluorescence spectrum varies according to the chemical polarity of the microenvironment into which the dye embeds. Nile Red spectroscopy identified histologically intact yet biochemically altered myelin in prelesioned tissues, including mouse white matter following subdemyelinating cuprizone intoxication, as well as normal-appearing white matter in multiple sclerosis brain. Nile Red spectroscopy offers a relatively simple yet highly sensitive technique for detecting subtle myelin changes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.