ArticlePlant physiology2021
The metabolic origins of non-photorespiratory CO2 release during photosynthesis: a metabolic flux analysis.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers, 1 of them a synthesis that pooled it.
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Who cites it
64 citing papers in PubMed, 1 synthesis or guideline pooled it, 121 citations in OpenAlex.
- Evaluating the contribution of plant metabolic pathways in the light to the ATP:NADPH demand using a meta-analysis of isotopically non-stationary metabolic flux analyses.Photosynthesis research · 2024Pooled it
- Chorismate and isochorismate turnover reveal hidden dynamics of aromatic metabolism in Arabidopsis leaves.The Plant cell · 2026Article
- The Contribution of Phloem Metabolism to Leaf Respiration: New Insights Into an Old Problem.Plant, cell & environment · 2026Article
- The Existence and Uniqueness of Solutions in the Leaf Photosynthesis-Transpiration-Stomatal Conductance Model for C3 Plants.Global change biology · 2026Article
- Understanding plant resilience by putting photosynthesis and photorespiration in the metabolic context.Planta · 2026Review
- Carbon Dioxide Release During Photosynthesis: Connecting Gas Exchange Behavior With Biochemistry.Plant, cell & environment · 2026Review
- C13MP, a method using C13 isotope labeling to measure plant photosynthetic rate precisely.Molecular breeding : new strategies in plant improvement · 2025Article
- Sub-optimal temperature leads to tighter coupling between photosynthetic electron transport and CO2 assimilation under fluctuating light in maize.Plant physiology · 2025Article
- Move over endosymbionts, peroxisomes pass electrons too.Biochemical Society transactions · 2025Review
- Time-resolved targeted metabolomics shows an abrupt switch from Calvin-Benson-Bassham cycle to tricarboxylic acid cycle when the light is turned off.Photosynthesis research · 2025Article
- Cryptic isoprene emission of soybeans.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Isoprene deters insect herbivory by priming plant hormone responses.Science advances · 2025Article
- Non-canonical plant metabolism.Nature plants · 2025Review
- Chlorine Modulates Photosynthetic Efficiency, Chlorophyll Fluorescence in Tomato Leaves, and Carbohydrate Allocation in Developing Fruits.International journal of molecular sciences · 2025Article
- The effects of photosynthetic rate on respiration in light, starch/sucrose partitioning, and other metabolic fluxes within photosynthesis.Scientific reports · 2025Article
- Metabolic flux analysis to increase oil in seeds.Plant physiology · 2025Review
- Modeling with uncertainty quantification reveals the essentials of a non-canonical algal carbon-concentrating mechanism.Plant physiology · 2025Article
- A Guide to Metabolic Network Modeling for Plant Biology.Plants (Basel, Switzerland) · 2025Review
- Article
- Rubisco supplies pyruvate for the 2-C-methyl-D-erythritol-4-phosphate pathway.Nature plants · 2024Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Respiration in the light (RL) releases CO2 in photosynthesizing leaves and is a phenomenon that occurs independently from photorespiration. Since RL lowers net carbon fixation, understanding RL could help improve plant carbon-use efficiency and models of crop photosynthesis. Although RL was identified more than 75 years ago, its biochemical mechanisms remain unclear. To identify reactions contributing to RL, we mapped metabolic fluxes in photosynthesizing source leaves of the oilseed crop and model plant camelina (Camelina sativa). We performed a flux analysis using isotopic labeling patterns of central metabolites during 13CO2 labeling time course, gas exchange, and carbohydrate production rate experiments. To quantify the contributions of multiple potential CO2 sources with statistical and biological confidence, we increased the number of metabolites measured and reduced biological and technical heterogeneity by using single mature source leaves and quickly quenching metabolism by directly injecting liquid N2; we then compared the goodness-of-fit between these data and data from models with alternative metabolic network structures and constraints. Our analysis predicted that RL releases 5.2 μmol CO2 g-1 FW h-1 of CO2, which is relatively consistent with a value of 9.3 μmol CO2 g-1 FW h-1 measured by CO2 gas exchange. The results indicated that ≤10% of RL results from TCA cycle reactions, which are widely considered to dominate RL. Further analysis of the results indicated that oxidation of glucose-6-phosphate to pentose phosphate via 6-phosphogluconate (the G6P/OPP shunt) can account for >93% of CO2 released by RL.
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