Evidence map›Paper›PMID 33591270›Full record

ArticleeLife2021

Proximity proteomics in a marine diatom reveals a putative cell surface-to-chloroplast iron trafficking pathway.

Jernej Turnšek, John K Brunson, Maria Del Pilar Martinez Viedma, Thomas J Deerinck, Aleš Horák, Miroslav Oborník, Vincent A Bielinski, Andrew Ellis Allen

Open access · goldAbstract read
In one paragraph

Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.8field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 29 citations in OpenAlex.

  1. Diel remodeling and cellular integration of the nitroplast.bioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. Genetic engineering in diatoms: advances and prospects.The Plant journal : for cell and molecular biology · 2025
    Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Iron uptake, transport and storage in marine brown algae.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2023
    Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Jernej TurnšekBiological and Biomedical Sciences, The Graduate School of Arts and Sciences, Harvard University, Cambridge, United States.ORCID 0000-0002-9056-3565
John K BrunsonMicrobial and Environmental Genomics, J. Craig Venter Institute, La Jolla, United States.
Maria Del Pilar Martinez ViedmaInformatics, J. Craig Venter Institute, La Jolla, United States.ORCID 0000-0002-8885-5008
Thomas J DeerinckNational Center for Microscopy and Imaging Research, University of California San Diego, La Jolla, United States.
Aleš HorákBiology Centre CAS, Institute of Parasitology, České Budějovice, Czech Republic.
Miroslav OborníkBiology Centre CAS, Institute of Parasitology, České Budějovice, Czech Republic.
Vincent A BielinskiSynthetic Biology and Bioenergy, J. Craig Venter Institute, La Jolla, United States.
Andrew Ellis AllenIntegrative Oceanography Division, Scripps Institution of Oceanography, University of California San Diego, La Jolla, United States.ORCID 0000-0001-5911-6081
J. Craig Venter Institute · USUniversity of South Bohemia in České Budějovice · CZHarvard University · USUniversity of California San Diego · US

Funding

The National Center for Microscopy and Imaging Research, a Community-wide Scientific ResourceR24GM137200 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ELLISMAN, MARK H · 2020 to 2022
$3.0M
Cellular and transcriptional regulation of the seafood toxin domoic acidF31ES030613 · NIEHS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI BRUNSON, JOHN KILPATRICK · 2019 to 2021
$100k
NIEHS NIH HHS F31 ES030613NIGMS NIH HHS R24 GM137200NIH HHS 1F31ES030613-01
6 · The paper itself

Abstract

Iron is a biochemically critical metal cofactor in enzymes involved in photosynthesis, cellular respiration, nitrate assimilation, nitrogen fixation, and reactive oxygen species defense. Marine microeukaryotes have evolved a phytotransferrin-based iron uptake system to cope with iron scarcity, a major factor limiting primary productivity in the global ocean. Diatom phytotransferrin is endocytosed; however, proteins downstream of this environmentally ubiquitous iron receptor are unknown. We applied engineered ascorbate peroxidase APEX2-based subcellular proteomics to catalog proximal proteins of phytotransferrin in the model marine diatom

Indexed as

Biological TransportCell MembraneChloroplastsDiatomsIronMultigene FamilyProteomicsTransferrinIronTransferrinAPEX2chloroplastdiatominfectious diseaseironmetal traffickingmicrobiologyphytotransferrinplant biology

Identifiers

PMID33591270
PMCPMC7972479
OpenAlexW3129792723

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.