ArticleAngewandte Chemie (International ed. in English)2021
Quantitative Assessment of the Physical Virus Titer and Purity by Ultrasensitive Flow Virometry.
Article in Angewandte Chemie (International ed. in English), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Rage against the mean: a perspective on measuring fitness of individual phage particles.Npj viruses · 2026Review
- Single-cell analysis reveals critical toxin/antitoxin ratio triggering persister resuscitation.Molecular systems biology · 2026Article
- Nano-Flow Cytometry-Guided Discrimination and Separation of Human Cytomegalovirus Virions and Extracellular Vesicles.Journal of extracellular vesicles · 2025Article
- Viral Membrane-Targeting Amphipathic Helical Peptide-Based Fluorogenic Probes for the Analysis of Infectious Titers of Enveloped Viruses.Analytical chemistry · 2025Article
- An automated and high-throughput approach for enhanced precision of adenoviral titering.Molecular therapy. Methods & clinical development · 2025Article
- Review
- Flow virometry: recent advancements, best practices, and future frontiers.Journal of virology · 2025Review
- Rapidly quantification of intact infectious H1N1 virus using ICA-qPCR and PMA-qPCR.Biosafety and health · 2024Article
- Article
- Review
- Rapid and specific detection of single nanoparticles and viruses in microfluidic laminar flow via confocal fluorescence microscopy.bioRxiv : the preprint server for biology · 2024Article
- Quantifying Plant Viruses: Evolution from Bioassay to Infectivity Dilution Curves along the Model of Tobamoviruses.Viruses · 2024Review
- High-Throughput Single-Cell Analysis of Nanoparticle-Cell Interactions.Trends in analytical chemistry : TRAC · 2023Article
- Recombinant Virus Quantification Using Single-Cell Droplet Digital PCR: A Method for Infectious Titer Quantification.Viruses · 2023Article
- Single-particle assessment of six different drug-loading strategies for incorporating doxorubicin into small extracellular vesicles.Analytical and bioanalytical chemistry · 2023Article
- A new method for quantitative analysis of M13 bacteriophage by atomic force microscopy.Synthetic and systems biotechnology · 2022Article
- Article
- Quantification of the Retention and Disassembly of Virus Particles by a PEI-Functionalized Microfiltration Membrane.ACS applied polymer materials · 2022Article
- Quantitative Assessment of the Physical Virus Titer and Purity by Ultrasensitive Flow Virometry.Angewandte Chemie (International ed. in English) · 2021Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid quantification of viruses is vital for basic research on viral diseases as well as biomedical application of virus-based products. Here, we report the development of a high-throughput single-particle method to enumerate intact viral particles by ultrasensitive flow virometry, which detects single viruses as small as 27 nm in diameter. The nucleic acid dye SYTO 82 was used to stain the viral (or vector) genome, and a laboratory-built nano-flow cytometer (nFCM) was employed to simultaneously detect the side-scatter and fluorescence signals of individual viral particles. Using the bacteriophage T7 as a model system, intact virions were completely discriminated from empty capsids and naked viral genomes. Successful measurement of the physical virus titer and purity was demonstrated for recombinant adenoviruses, which could be used for gene delivery, therapeutic products derived from phage cocktails, and infected cell supernatants for veterinary vaccine production.
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