Evidence map›Paper›PMID 33587303›Full record

ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2021

Functional contribution of DCLKs in sea urchin development.

Derek Xu, Florence D M Wavreil, Ashley Waldron, Mamiko Yajima

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Derek XuMCB Department, Brown University, Providence, Rhode Island, USA.
Florence D M WavreilMCB Department, Brown University, Providence, Rhode Island, USA.
Ashley WaldronMCB Department, Brown University, Providence, Rhode Island, USA.
Mamiko YajimaMCB Department, Brown University, Providence, Rhode Island, USA.ORCID 0000-0002-5613-1005
Brown University · US

Funding

Localized mRNA translation on the spindle -an essential mechanism for embryonic cell regulationR01GM126043 · NIGMS · BROWN UNIVERSITY · PI YAJIMA, MAMIKO · 2018 to 2022
$2.0M
NIGMS NIH HHS R01 GM126043
6 · The paper itself

Abstract

backgroundDoublecortin-like kinase1 and 2 (DCLKs) are protein Ser/Thr kinases important for neuronal development. More recently, they are also reported to regulate plasticity such as cell proliferation and differentiation of stem cells and cancer cells, but the details of their functions in this biological context are still unclear. With an attempt to reveal the functions of DCLKs in plasticity regulation, we here used the sea urchin embryo that undergoes highly regulative development as an experimental model.

resultsWe found that both the transcripts and the proteins of DCLKs are uniformly present during early embryogenesis and with some enrichment in mesenchymal cells after gastrula stage. Knockdown of DCLKs induced general developmental delay and defects at day 2. Further, the damage on the embryo/larva induced ectopic expression of DCLKs in the ectoderm where the damage was most severe. Under a tumor-prone or -suppressive condition, DCLKs expression was upregulated or downregulated, respectively, after damage. In both cases, the embryos showed severe developmental defects.

conclusionsTaken together, a transient upregulation of DCLKs appears to be involved in a damage response both during normal and abnormal development, and which could result in different phenotypes in a context dependent manner.

Indexed as

Gene Expression Regulation, DevelopmentalAnimalsCell DifferentiationDoublecortin-Like KinasesEmbryonic DevelopmentEmbryo, NonmammalianSea UrchinsDoublecortin-Like Kinasescell differentiationDoublecortin like kinaseembryonic developmentplasticity regulationsea urchin

Identifiers

PMID33587303
PMCPMC8439984
OpenAlexW3132233323

What OpenQuestion holds

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LicenceTDM
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.