ArticleBiotechnology and applied biochemistry2022
Enhancement and mechanisms of MC3T3-E1 osteoblast-like cell adhesion to albumin through calcium exposure.
Article in Biotechnology and applied biochemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 26 citations in OpenAlex.
- Osteoblast and osteoclast responses to chelate-setting calcium phosphate cements with different solubilities.Biomaterials and biosystems · 2026Article
- Developing Multimodal Cu-TCP@PCL Periosteal Patch with Modulated Morphogenesis and Osteogenic Enhancement for Bone Tissue-Engineering Application.International journal of nanomedicine · 2026Article
- Potential of zoledronate for treating diffuse sclerosing osteomyelitis of the mandible in adult patients.Clinical rheumatology · 2025Article
- Addressing the challenges of infectious bone defects: a review of recent advances in bifunctional biomaterials.Journal of nanobiotechnology · 2025Review
- POs-Ca Drives Osteogenic Differentiation of Human Dental Pulp Stem Cells Via AMPK-Dependent Autophagy Activation and Reciprocal Calcium-Autophagy Crosstalk.Stem cells international · 2025Article
- The predictive value of albumin to alkaline phosphatase ratio for vertebral refractures in postmenopausal women.Journal of bone and mineral metabolism · 2024Article
- Mesoporous Oxidized Mn-Ca Nanoparticles as Potential Antimicrobial Agents for Wound Healing.Molecules (Basel, Switzerland) · 2024Article
- Evaluation of Serum Albumin-Coated Bone Allograft for Bone Regeneration: A Seven-Year Follow-Up Study of 26 Cases.International journal of molecular sciences · 2023Article
- Delivery of Bioactive Albumin from Multi-Functional Polyampholyte Hydrogels.Journal of applied polymer science · 2022Article
- Albumin as a Biomaterial and Therapeutic Agent in Regenerative Medicine.International journal of molecular sciences · 2022Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Serum albumin is the most prominent protein in blood, and it aids in bone fracture healing, though the manner through which enhanced healing occurs is not well understood. This study investigates the influence of calcium on the bioactivity of albumin due to the prevalence of calcium at bone injury sites. Bovine serum albumin (BSA) was exposed to varying concentrations of calcium, adsorbed to tissue culture polystyrene, and the subsequent BSA-coated surfaces were evaluated with calcium titration, and cell adhesion, viability, and binding inhibition studies. Calcium-modified BSA improved overall MC3T3-E1 osteoblast-like cell adhesion, although high calcium concentrations induced cell death. Inhibiting specific integrins revealed that without calcium exposure, cell binding to BSA was primarily mediated by integrins that typically bind to the GFOGER sequence of collagen. As calcium exposure increases, the primary binding interaction transitioned to integrins known to bind RGD. However, cell binding to calcium-modified BSA was not completely eliminated during the inhibition studies indicating additional unidentified binding interactions occur. Overall, these results suggest that the exposure to calcium induces conformational changes that affect the cell-binding bioactivity of BSA, which may explain the beneficial impact of albumin in bone tissue.
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