ArticleG3 (Bethesda, Md.)2021
Whole-genome assembly of Culex tarsalis.
Article in G3 (Bethesda, Md.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Phylogeography and Climate-Driven Distribution of Culex tarsalis (Diptera: Culicidae): Implications for the Global Spread of West Nile Virus.Acta parasitologica · 2026Article
- Behavioral heterogeneity in host seeking and post-feeding suppression among disease vector mosquitoes.Communications biology · 2026Article
- Virome profiling of Culex tarsalis through small RNA-seq: A challenge of suboptimal samples.PLoS neglected tropical diseases · 2025Article
- Climate Adaptation and Genetic Differentiation in the Mosquito Species Culex tarsalis.Genome biology and evolution · 2025Article
- Behavioral heterogeneity in host-seeking and post-feeding suppression among disease vector mosquitoes.bioRxiv : the preprint server for biology · 2025Article
- A single-cell atlas of the Culex tarsalis midgut during West Nile virus infection.PLoS pathogens · 2025Article
- A single-cell atlas of thebioRxiv : the preprint server for biology · 2024Article
- The chromosome-scale genome assembly for the West Nile vector Culex quinquefasciatus uncovers patterns of genome evolution in mosquitoes.BMC biology · 2024Article
- De novo genome assembly of the invasive mosquito species Aedes japonicus and Aedes koreicus.Parasites & vectors · 2023Article
- Article
- Culex tarsalis Is a Competent Host of the Insect-Specific Alphavirus Eilat Virus (EILV).Journal of virology · 2023Article
- Identification of MicroRNAs in the West Nile Virus Vector Culex tarsalis (Diptera: Culicidae).Journal of medical entomology · 2023Article
- An insight into the female and male Sabethes cyaneus mosquito salivary glands transcriptome.Insect biochemistry and molecular biology · 2023Article
- Elucidating the role of dsRNA sensing and Toll6 in antiviral responses ofFrontiers in cellular and infection microbiology · 2023Article
- Article
- Mosquito saliva enhances virus infection through sialokinin-dependent vascular leakage.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A nickase Cas9 gene-drive system promotes super-Mendelian inheritance in Drosophila.Cell reports · 2022Article
- Extending and Running the Mosquito Small RNA Genomics Resource Pipeline.Methods in molecular biology (Clifton, N.J.) · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The mosquito, Culex tarsalis, is a key vector in the western United States due to its role in transmission of zoonotic arboviruses that affect human health. Extensive research has been conducted on Cx. tarsalis ecology, feeding behavior, vector competence, autogeny, diapause, genetics, and insecticide resistance. Population genetic analyses in the western U.S. have identified at least three genetic clusters that are geographically distinct. However, in-depth genetic studies have been hindered by the lack of a reference genome. In this study, we present the first whole-genome assembly of this mosquito species (CtarK1) based on PacBio HiFi reads from high-molecular-weight DNA extracted from a single male. The CtarK1 assembly is 790 Mb with an N50 of 58 kb, which is 27% larger than Culex quinquefasciatus (578 Mb). This difference appears to be mostly composed of transposable elements. To annotate CtarK1, we used a previously assembled Cx. tarsalis transcriptome and approximately 17,456 protein genes from Cx. quinquefasciatus (N = 17,456). Genome completeness was assessed using the Benchmarking Universal Single-Copy Orthologs (BUSCO) tool, which identified 84.8% of the 2799 Dipteran BUSCO genes. Using a Bayesian phylogeny based on mitochondrial genomes, we place Cx. tarsalis in the context of other mosquito species and estimate the divergence between Cx. tarsalis and Cx. quinquefasciatus to be between 15.8 and 22.2 million years ago (MYA). Important next steps from this work include characterizing the genetic basis of diapause and sex determination in Culex mosquitoes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.